Salivary IgA antibody to oral streptococcal antigens in predentate infants.
Salivary IgA antibody to oral streptococcal antigens in predentate infants.
复制标题
未出牙婴儿的唾液 IgA 抗体针对口腔链球菌抗原。
DOI：
10.1111/j.1399-302x.1990.tb00228.x
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发表时间：
1990
影响因子：
--
通讯作者：
Taubman,MA
中科院分区：
文献类型：
--
作者：
Smith,DJ;King,WF;Taubman,MA
对17例3 ~ 27周龄婴儿的53份完整唾液进行了口腔链球菌免疫球蛋白a抗体的分析。在12周龄时，用酶联免疫吸附法检测了78%的全唾液对米特细胞链球菌的抗体活性。唾液经Superose 6凝胶过滤后，ELISA检测该抗体活性与聚合IgA相关。免疫印迹法检测轻度链球菌和唾液链球菌培养上清的IgA抗体。41例，91%的唾液样本含有IgA抗体，免疫印迹分析与s。salivariusandS。分别培养上清液。口服链球菌抗原制备中出现IgA抗体反应性的最小婴儿为5周大。从这些婴儿的口腔中分离出各自的链球菌种类后，用Western blot检测到对两种细菌培养上清的唾液IgA抗体。在不同婴儿的唾液形成的模式中观察到一些异质性。这些结果表明，唾液IgA抗体反应可能在出生后第一个月通过口腔定植（S. mitis， S. salivarius）诱导。
Salivary IgA antibodies to oral streptococci which colonize the infant oral cavity soon after birth were analyzed in 53 whole salivas of 17 infants who were between three and 27 weeks old. Antibody activities toStreptococcus mitiscells were detected by enzyme linked immunosorbant assay in 78% of the whole salivas by the twelfth week of age. This antibody activity was associated with polymeric IgA as determined after gel filtration of salivas on Superose 6, followed by ELISA. Western blot analyses were used to detect IgA antibodies toStreptococcus mitisandStreptococcus salivariusculture supernatants. Forty one, and 91% of saliva samples contained IgA antibody which reacted in Western blot analyses withS. salivariusandS. mitisculture supernatants, respectively. The youngest infant to show reactive IgA antibody with either oral streptococcal antigen preparation was five weeks old. Salivary IgA antibody to either bacterial culture supernatant was detected in Western blot only after the isolation of the respective streptococcal species from the oral cavity of these young infants. Some heterogeneity was observed among patterns developed with salivas from different infants. These results suggest that salivary IgA antibody responses may be induced by oral colonization (S. mitis, S. salivarius) by the end of the first month of life.