Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene
Identification of polymorphism and association analysis with reproductive traits in the porcine RNF4 gene
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DOI：
10.1016/j.anireprosci.2008.01.020
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发表时间：
2009-02-01
影响因子：
2.2
通讯作者：
Xiong, Y. Z.
中科院分区：
文献类型：
--
作者：
Niu, B. Y.;Ye, L. Z.;Xiong, Y. Z.
Ring finger protein 4基因（RNF 4）可能在胚胎生殖细胞发育、卵母细胞和颗粒细胞成熟过程中发挥重要作用，是潜在的生殖性状候选基因之一。本研究分离了猪RNF 4基因的全编码序列，在内含子5上发现了一个单核苷酸多态性（SNP：TIC），并建立了PCR-SacII-RFLP基因分型方法。在三个具有不同遗传背景的人群中评估了这种SNP与生殖性状的关联。一个是中国清平母猪。另一组是来自长白、大白色、中国通城和/或中国梅山的杂交母猪（DIV系）。第三组为大白色×梅山（LW × M）F，屠宰群体。统计分析表明，在第一胎，RNF 4基因型与清平母猪和DIV系母猪的繁殖性状差异不显著。在第二窝和以后的窝里。CC型清平猪群的产仔数（+1.74头）和活产数（+2.02头）均高于TT型（P
The ring finger protein 4 gene (RNF4), which might play a role in fetal germ cell development as well as in oocyte and granulosa cell maturation, was one of the potential candidate genes for reproductive traits. In the present work, we isolated the complete coding sequence of porcine RNF4 gene, identified a single nucleotide polymorphism (SNP: TIC) in intron5, and developed a PCR-SacII-RFLP genotyping assay. Association of this SNP with reproductive traits was assessed in three populations with diverse genetic backgrounds. One was Chinese Qingping sows. Another was consisted of crossbred sows derived from Landrace, Large White, Chinese Tongcheng and/or Chinese Meishan (Line DIV). The third is Large White x Meishan (LW x M) F, slaughtered population. Statistical analysis demonstrated that, in the first parity, the difference between RNF4 genotypes and reproductive traits of both Qingping and Line DIV sows was not significant. In the second and subsequent litters. CC animals in Qingping population had more piglets born (+1.74 piglets) and piglets born alive (+2.02 piglets) than sows with the TT genotype (P