Effects of alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor or ornithine decarboxylase, on testosterone-induced regeneration of prostate and seminal vesicle in castrated rats.
Effects of alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor or ornithine decarboxylase, on testosterone-induced regeneration of prostate and seminal vesicle in castrated rats.
复制标题
α-二氟甲基鸟氨酸（一种酶激活的不可逆抑制剂或鸟氨酸脱羧酶）对睾酮诱导的去势大鼠前列腺和精囊再生的影响。
作者：
C. Danzin;M. Jung;N. Claverie;J. Grove;A. Sjoerdsma;J. Koch
1.成年大鼠去势后，前列腺腹侧和精囊中多胺(腐胺、亚精胺和精胺)含量及RNA和DNA含量显著减少。2.去势7天后，每日注射丙酸睾丸酮的大鼠精囊中多胺和核酸含量的增加速度快于前列腺腹侧。3.雄激素处理7天后，精囊多胺和核酸含量显著高于正常动物。在接下来的4天继续治疗期间，核酸含量恢复到正常值，但多胺含量不变。在前列腺中，雄激素治疗使多胺和核酸含量增加到正常值，但不高于正常值。4.反复给予酶激活的不可逆鸟氨酸脱羧酶抑制剂α-二氟甲基鸟氨酸，可完全阻断睾酮引起的前列腺腹侧腐胺和精囊腐胺的增加。它们显著地减缓了精胺在前列腺腹侧的积累和精胺在精囊中的积累。α-二氟甲基鸟氨酸还延缓了睾酮诱导的RNA在前列腺腹侧的积聚。然而，在这两个器官中，多胺和核酸的积累之间没有明显的相关性。5.α-二氟甲基鸟氨酸显著延缓睾酮诱导的前列腺增重，但对精囊无明显影响。细胞学研究表明，这种对前列腺的影响是由于抑制了雄激素诱导的前列腺腺泡分泌内容的恢复。
1. Castration of adult rats markedly decreases the amounts of polyamines (putrescine, spermidine and spermine) and of RNA and DNA in the ventral prostate and the seminal vesicle. 2. Daily injections of testosterone propionate to rats castrated 7 days previously increase polyamine and nucleic acid contents more rapidly in the seminal vesicle than in the ventral prostate. 3. After 7 days of androgen treatment, polyamine and nucleic acid contents of the seminal vesicle are significantly higher than those of intact animals. Nucleic acid, but not polyamine, contents return to normal values during the next 4 days of continued treatment. In the prostate, androgen treatment increases polyamine and nucleic acid contents to, but not above, normal values. 4. Repeated doses of alpha-difluoromethylornithine, a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase, totally blocked the testosterone-induced increase of putrescine and spermidine in the ventral prostate and of putrescine in the seminal vesicle. They slowed significantly the accumulation of spermine in the ventral prostate and of spermidine in the seminal vesicle. alpha-Difluoromethylornithine also retarded the testosterone-induced accumulation of RNA in the ventral prostate. However, no clear correlation was apparent between accumulation of polyamines and of nucleic acids in the two organs. 5. alpha-Difluoromethylornithine markedly slows the testosterone-induced weight gain of the prostate, but not of the seminal vesicle. Cytological studies suggest that this effect on the prostate is due to inhibition of the androgen-induced restoration of the secretion content of prostatic acini.