Evaluation of seasonal influenza vaccinese for H1N1pdm09 and type B viruses based on a replication-incompetent PB2-KO virus.
Evaluation of seasonal influenza vaccinese for H1N1pdm09 and type B viruses based on a replication-incompetent PB2-KO virus.
复制标题
基于无复制能力的 PB2-KO 病毒对 H1N1pdm09 和 B 型病毒的季节性流感疫苗进行评估。
DOI：
10.1016/j.vaccine.2017.02.041
复制
发表时间：
2017
期刊：
影响因子：
5.5
通讯作者：
Kawaoka Y.
中科院分区：
文献类型：
--
作者：
Ui H;Yamayoshi S;Uraki R;Kiso M;Oishi K;Murakami S;Mimori S;Kawaoka Y.
接种疫苗是预防人类感染流感病毒的第一道防线。虽然灭活疫苗和减毒活疫苗是可用的，但每种疫苗在免疫原性和安全性方面都有缺点。为了克服这些问题，我们的研究小组已经开发出一种只能在PB 2表达细胞中复制的复制缺陷型PB 2敲除（PB 2-KO）流感病毒。在这里，我们产生了PB 2-KO病毒具有血凝素（HA）和神经氨酸酶（NA）节段从H1N1 pdm 09或B型病毒，并测试其疫苗的潜力。两种PB 2-KO病毒在PB 2表达细胞中有效繁殖，并如预期那样表达嵌合HA。在用病毒免疫的小鼠中检测到病毒特异性IgG和伊加抗体，并且免疫小鼠在病毒攻击后显示出较轻的临床体征和/或呼吸道中较低的病毒复制水平。我们的研究结果表明，这些PB 2-KO病毒具有作为候选疫苗的潜力。
Vaccination is the first line of protection against influenza virus infection in humans. Although inactivated and live-attenuated vaccines are available, each vaccine has drawbacks in terms of immunogenicity and safety. To overcome these issues, our group has developed a replication-incompetent PB2-knockout (PB2-KO) influenza virus that replicates only in PB2-expressing cells. Here we generated PB2-KO viruses possessing the hemagglutinin (HA) and neuraminidase (NA) segments from H1N1pdm09 or type B viruses and tested their vaccine potential. The two PB2-KO viruses propagated efficiently in PB2-expressing cells, and expressed chimeric HA as expected. Virus-specific IgG and IgA antibodies were detected in mice immunized with the viruses, and the immunized mice showed milder clinical signs and/or lower virus replication levels in the respiratory tract upon virus challenge. Our results indicate that these PB2-KO viruses have potential as vaccine candidates.