The morphology of GM1x/SM0.6-x/Chol0.4 planar bilayers supported on SiO2 surfaces
The morphology of GM1x/SM0.6-x/Chol0.4 planar bilayers supported on SiO2 surfaces
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DOI：
10.1016/j.cplett.2008.05.097
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发表时间：
2008-07-20
影响因子：
2.8
通讯作者：
Urisu, Tsuneo
中科院分区：
文献类型：
--
作者：
Mao, Yanli;Tero, Ryugo;Urisu, Tsuneo
神经节苷脂GM 1（GM 1）、鞘磷脂（SM）和胆固醇（Chol）是质膜筏的主要脂质成分。用原子力显微镜和荧光显微镜研究了不同GM_1/SM（x = 0-0.25）比例下，GM_1（x）/SM（0.6）（x）/Chol（0.4）SPBs在SiO_2表面的形貌.通过SM和Chol之间的氢键作用和疏水相互作用，以及在Ca（2+）存在的条件下GM 1头基之间的氢键作用和空间效应，以及在Ca（2+）不存在的条件下GM 1头基负电荷之间的静电排斥，定性地解释了这种独特的形态变化. (C)2008 Elsevier B. V.保留所有权利。
Ganglioside GM1 (GM1), sphingomyelin (SM) and cholesterol (Chol) are dominant lipid components of rafts in plasma membranes. The morphology of GM1(x)/SM(0.6) (x)/Chol(0.4) SPBs on SiO(2) surfaces has been studied by atomic force microscopy and fluorescence microscopy at various ratios of GM1/SM (x = 0-0.25). The unique changes in morphology depending on the GM1 concentrations are qualitatively explained by hydrogen bonding and the hydrophobic interactions between SM and Chol, and by hydrogen bonding and the steric effects between bulky GM1 headgroups under Ca(2+) existing conditions and the electrostatic repulsion between the negative charges of GM1 headgroups under Ca(2+) nonexisting conditions. (C) 2008 Elsevier B.V. All rights reserved.