Expression and Localization of Aquaporins in the Kidney of the Musk Shrew (Suncus murinus)
Expression and Localization of Aquaporins in the Kidney of the Musk Shrew (Suncus murinus)
复制标题
DOI：
10.1369/jhc.7a7281.2007
复制
发表时间：
2008-01
影响因子：
3.2
通讯作者：
Seishi Maeda;S. Kuwahara;Hisao Itô;Koichii Tanaka;T. Hayakawa;M. Seki
中科院分区：
文献类型：
--
作者：
Seishi Maeda;S. Kuwahara;Hisao Itô;Koichii Tanaka;T. Hayakawa;M. Seki
采用免疫组织化学方法研究了水通道蛋白（AQP）家族成员（AQP 1、2、3、4和5）在麝香鼩肾脏中的表达和定位。AQP 1表达于近端小管和Henle袢的细支。AQP 1是近端肾单位中唯一表达的水通道，表明AQP 1可能是近端肾单位中独立的水转运蛋白。AQP 2和AQP 5定位于皮质-髓质集合管（CD）细胞的顶端细胞质，AQP 3和AQP 4定位于皮质-髓质CD细胞的基底面。AQP 3在皮质细胞中的表达较弱，而AQP 4在整个CD中呈强阳性。提示CD是肾单位水重吸收的主要环节，并受AQPs的调节。事实上，顶端水运输的CD细胞的麝香鼩可能是由AQP 2和AQP 5。该动物中AQP的特征性表达模式为阐明哺乳动物肾脏中AQP对水重吸收的调节提供了一种新的动物模型。
Expression and localization of members of the aquaporin (AQP) family (AQP1, 2, 3, 4, and 5) in the kidney of the musk shrew (Suncus murinus) was examined by immunohistochemistry. AQP1 was expressed in the proximal tubules and in the thin limb of the loops of Henle. AQP1 was the only water channel expressed in the proximal nephron examined, indicating that AQP1 may be an independent water transporter in the proximal nephron. AQP2 and AQP5 were localized to the apical cytoplasm of the cortical to medullary collecting duct (CD) cells and AQP3 and AQP4 were localized to the basal aspect of the cortical to medullary CD cells. AQP3 expression was weaker in the cortical cells compared with the medullary cells, whereas AQP4 was strongly positive throughout the CD. These indicate that the CD is the main water reabsorption segment of the nephron and is regulated by AQPs. Indeed, apical water transport of CD cells of the musk shrew may be controlled by both AQP2 and AQP5. The characteristic expression pattern of the AQPs in this animal provides a novel animal model for elucidating the regulation of water reabsorption by AQPs in the mammalian kidney.