Effects of Exogenous L-Asparagine on Poplar Biomass Partitioning and Root Morphology.
Effects of Exogenous L-Asparagine on Poplar Biomass Partitioning and Root Morphology.
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外源L-天冬酰胺对杨树生物量分配和根系形态的影响
DOI：
10.3390/ijms232113126
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发表时间：
2022-10-28
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
天冬酰胺（L-Asparagine，Asn）具有较高的氮碳比（2：4）和稳定性，被认为是植物体内最经济的氮素储存和运输分子之一。虽然它的内部功能已被解决，外源Asn在植物中的生物学作用仍然是难以捉摸的。在本研究中，不同浓度（0.5，1，2，或5 mM）的Asn添加到氮缺乏水培溶液中的白杨'南林895'。形态计量学分析表明，Asn处理显著促进了杨树的白杨高、生物量和光合活性。此外，Asn处理显著改变了氨基酸含量、全氮和全碳含量以及硝酸盐和氨含量。此外，外源Asn引起的根生长抑制，伴随着复杂的基因转录模式和酶的活性与N和C代谢的变化。结合植株表型和生理生化指标分析表明，白杨具有吸收利用外源Asn的能力，且具有剂量依赖性。这为研究不同形态氮和Asn浓度对白杨根、冠生长和功能的影响以及Asn在蛋白质稳态调节中的作用提供了有价值的信息和见解。
L-Asparagine (Asn) has been regarded as one of the most economical molecules for nitrogen (N) storage and transport in plants due to its relatively high N-to-carbon (C) ratio (2:4) and stability. Although its internal function has been addressed, the biological role of exogenous Asn in plants remains elusive. In this study, different concentrations (0.5, 1, 2, or 5 mM) of Asn were added to the N-deficient hydroponic solution for poplar ‘Nanlin895’. Morphometric analyses showed that poplar height, biomass, and photosynthesis activities were significantly promoted by Asn treatment compared with the N-free control. Moreover, the amino acid content, total N and C content, and nitrate and ammonia content were dramatically altered by Asn treatment. Moreover, exogenous Asn elicited root growth inhibition, accompanied by complex changes in the transcriptional pattern of genes and activities of enzymes associated with N and C metabolism. Combined with the plant phenotype and the physiological and biochemical indexes, our data suggest that poplar is competent to take up and utilize exogenous Asn dose-dependently. It provides valuable information and insight on how different forms of N and concentrations of Asn influence poplar root and shoot growth and function, and roles of Asn engaged in protein homeostasis regulation.
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影响因子：
4.3
作者：
BROUQUISSE, R;JAMES, F;RAYMOND, P
通讯作者：
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影响因子：
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