The correlation between denaturation of glyceraldehyde-3-phosphate dehydrogenase and inactivation of <i>Saccharomyces pastorianus</i> cells by pressurized carbon dioxide microbubbles
The correlation between denaturation of glyceraldehyde-3-phosphate dehydrogenase and inactivation of <i>Saccharomyces pastorianus</i> cells by pressurized carbon dioxide microbubbles
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加压二氧化碳微泡对3-磷酸甘油醛脱氢酶变性与巴斯德酵母细胞灭活的相关性
DOI：
10.1093/lambio/ovad043
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发表时间：
2023
影响因子：
2.4
通讯作者：
Odake Sachiko
中科院分区：
文献类型：
--
作者：
Takahashi Konomi;Kobayashi Fumiyuki;Narai-Kanayama Asako;Odake Sachiko
为探讨加压二氧化碳微泡（CO2 MB）处理对酵母细胞甘油醛-3-磷酸脱氢酶（GAPDH）失活与巴氏杀菌的关系，采用了一种新的酵母细胞保存试验方法。对CO_2 MB处理后的酵母细胞进行了细胞恢复试验，并对CO_2 MB灭活酵母细胞GAPDH的处理条件进行了研究。每个种群S.在35 °C处理3 min（MB 35 -3和MB 35 -5）或在40°C和45°C处理1 min和3 min（MB 40 -1、MB 40 -3和MB 45 -1）显著降低了在25°C下处理48、96和144 h的毕赤酵母的生长。在储存试验中，在25 ℃下储存144 h后未观察到处理细胞的恢复。GAPDH在S.用十二烷基硫酸钠聚丙烯酰胺凝胶电泳检测与贮存试验相同处理引起的pastorianus细胞。而MB 35 -1、MB 35 -3和MB 40 -1处理的酶活性显著高于未处理的酶活性，MB 35 -5、MB 40 -3和MB 45 -1处理的酶活性低于未处理的酶活性。因此，GAPDH变性而非活性与S的失活有关。pastorianuscells.
For the purpose of clarifying the relationship between pasteurization and inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) inSaccharomyces pastorianuscells induced by pressurized carbon dioxide microbubbles (CO2MB) treatment, a storage test ofS. pastorianuscells after CO2MB treatment was conducted to ascertain their recovery, and the treatment condition in the inactivation of GAPDH inS. pastorianuscells by CO2MB was investigated. Each population ofS. pastorianusfor 48, 96, and 144 h at 25°C was decreased significantly by CO2MB treatment at 35°C for 3 min (MB35-3 and MB35-5) or at 40°C and 45°C for 1 and 3 min (MB40-1, MB40-3, and MB45-1). In the storage test, recovery of treated cells was not observed after storage for 144 h at 25°C. The denaturation of GAPDH in theS. pastorianuscells caused by the same treatment as the storage test was detected by using sodium dodecyl sulphate polyacrylamide gel electrophoresis. While the activities at MB35-1, MB35-3, and MB40-1 were significantly higher than those at non-treatment, and those at MB35-5, MB40-3, and MB45-1 were lower. Therefore, GAPDH denaturation, but not the activity, was associated with the inactivation ofS. pastorianuscells.