Biocatalysis and Agricultural Biotechnology
Biocatalysis and Agricultural Biotechnology
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通讯作者：
Bastien Cochereau;T. R. D. Pont;Y. Pouchus;Deniz Tasdemir;L. Meslet-Cladière;C. Roullier
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作者：
Bastien Cochereau;T. R. D. Pont;Y. Pouchus;Deniz Tasdemir;L. Meslet-Cladière;C. Roullier
这项研究首次报道了使用钒氯过氧化物酶（vCPO）酶来增加海洋真菌提取物的化学多样性，以产生新的天然化学实体。使用的vCPO是来自海洋真菌Hortaea werneckii（Hw vCPO）的重组蛋白。它催化次卤酸（HOX）的形成，这是一种高活性化合物，可以与富电子底物反应。在这里，从不同的海洋真菌菌株（扩展青霉，Aspergillus pseudoglaucus，木霉属和Hortaea werneckii）获得的四种真菌提取物进行了研究，以提高其化学多样性。代谢组学研究表明，酶法处理扩展青霉和黄芪提取物的效果优于酶法处理。pseudoglaucus通过增加卤代分子的数量显著提高了化学多样性。实际上，与阴性对照相比，在提取物的ESI-MS图谱中分别检测到5.07和6.65倍的卤代离子。产生的新化学物质允许识别新的溴化化合物，其中一种被进一步纯化并表征为12-溴-communesin A（2）。与communesin A（1）相比，这种新化合物对耐甲氧西林金黄色葡萄球菌表现出中等的抗菌活性（IC 50为62 μ M）。这项研究清楚地表明，vCPO酶的就业是一个有前途的和环境友好的战略，以提高天然提取物的化学多样性。
This study reports for the first time the use of a vanadium chloroperoxidase (vCPO) enzyme to increase the chemical diversity of marine fungal extracts to generate new natural chemical entities. The vCPO used is a recombinant protein from the marine fungus Hortaea werneckii ( Hw vCPO). It catalyzes the formation of hypohalous acid (HOX), a highly reactive compound that can react with electron-rich substrates. Here, four fungal extracts obtained from different marine fungal strains ( Penicillium expansum , Aspergillus pseudoglaucus , Trichoderma sp. and Hortaea werneckii ) were investigated for enhancement of their chemical diversity. The metabolomic study showed that the enzymatic treatment of extracts of P. expansum and A. pseudoglaucus significantly boosted the chemodiversity by increasing the number of halogenated molecules. Indeed, respectively 5.07 and 6.65 times more halogenated ions were detected in ESI-MS profile of the extracts compared to negative controls. The new chemistry generated allowed the identification of new brominated compounds, one of which was further purified and characterized as 12-bromo-communesin A ( 2 ). This new compound, in contrast to communesin A ( 1 ), exhibited moderate antimicrobial activity on the methillicin-resistant Staphylococcus aureus (IC 50 of 62 μ M). This study has clearly demonstrated the employment of the vCPO enzyme to be a promising and environmentally friendly strategy to enhance the chemical diversity of natural extracts.