Fluorescent dye prelabelled microglial cells migrate into organotypic hippocampal slice cultures and ramify
Fluorescent dye prelabelled microglial cells migrate into organotypic hippocampal slice cultures and ramify
复制标题
DOI：
10.1111/j.1460-9568.1997.tb01436.x
复制
发表时间：
1997-04-01
影响因子：
3.4
通讯作者：
Nitsch, R
中科院分区：
文献类型：
--
作者：
Hailer, NP;Heppner, FL;Nitsch, R
具有其特征性分枝形态的小胶质细胞仅在健康CNS组织中发现，而各种病理与变形虫样巨噬细胞样细胞的发生相关。这仍然是一个讨论的问题，无论是阿米巴样细胞是血液来源的巨噬细胞，或是否在形态学上的特征性变化，反映了先前分支的小胶质细胞的激活，发生。从健康大鼠脑中获得的解离的小胶质细胞培养物中的细胞，不可避免地发展这种变形虫形态，用荧光染料标记并转移到器官型海马切片培养物上。具有变形虫形态的预标记细胞侵入这些切片培养物，并在体外培养9天后逐渐转化为高度分枝的细胞。我们的研究结果加强了这一假设，即所观察到的变形虫和分支细胞属于一个单一的人口的小胶质细胞，出现不同的形态取决于刺激的存在下提供的中枢神经系统微环境。小胶质细胞明显地以不同的形状出现，并且可以从免疫静止模式切换到激活模式，反之亦然。
Microglial cells with their characteristic ramified morphology are exclusively found in healthy CNS tissue, whereas various pathologies are associated with the occurrence of amoeboid, macrophage-like cells. It is still a matter of discussion whether amoeboid cells are blood-derived macrophages, or whether a characteristic change in morphology, reflecting activation of previously ramified microglia, takes place. Cells in dissociated microglia culture obtained from healthy rat brains, inevitably developing this amoeboid morphology, were labelled with a fluorescent dye and transferred onto organotypic hippocampal slice cultures. Prelabelled cells with amoeboid morphology invaded these slice cultures and had, after 9 days in vitro, gradually transformed into highly ramified cells. Our findings strengthen the hypothesis that the observed amoeboid and ramified cells belong to a single population of microglia, appearing with different morphologies depending on the presence of stimuli provided by the CNS microenvironment. Microglial cells obviously appear in different shapes and can switch from immunologically resting to activated modes and vice verse.