Quantification of genistein and genistin in soybeans and soybean products
Quantification of genistein and genistin in soybeans and soybean products
复制标题
DOI：
10.1016/0278-6915(96)87355-8
复制
发表时间：
1996-05-01
影响因子：
4.3
通讯作者：
Wakabayashi, K
中科院分区：
文献类型：
--
作者：
Fukutake, M;Takahashi, M;Wakabayashi, K
已经表明，染料木黄酮（genistein）可能作为人类癌症的化学预防剂具有某些作用。采用高效液相色谱法对日本人在大豆和相关豆制品中摄入的染料木黄酮及其β-葡萄糖苷结合物染料木苷的水平进行定量，以估计这些化合物的每日摄入量。大豆、大豆坚果和大豆粉中染料木黄酮和染料木苷的含量范围分别为4.6至18.2和200.6至968.1微克/克食物。豆浆和豆腐（豆腐）的值分别为1.9至13.9和94.8至137.7微克/克食物。发酵豆制品、豆酱和纳豆中的金雀异黄素和金雀异黄素的含量分别为38.5至229.1微克/克食物和71.7至492.8微克/克食物。因此，发酵豆制品中染料木黄酮的含量高于大豆和大豆制品如豆奶和豆腐。从这些观察结果可以看出，染料木苷的β-糖基键在发酵过程中被微生物裂解以产生染料木黄酮，从而产生纳豆和纳豆。酱料也被发现含有这两种黄酮，但含量低于番茄酱和纳豆。根据这些大豆和相关产品的年平均消费量数据，计算出日本人每天摄入的染料木黄酮和染料木苷分别为1.5-4.1和6.3-8.3毫克/人。这些水平远远高于美国人或西欧人，他们的乳腺癌，结肠癌和前列腺癌的死亡率高于日本人。(C)1996年Elsevier Science Ltd.
It has been suggested that the isoflavone, genistein,, may have some role as a chemopreventive agent against cancer in humans. Levels of genistein and its beta-glucoside conjugate, genistin, ingested in soybeans and related bean products by the Japanese were quantified by HPLC, to estimate daily intake of these compounds. Amounts of genistein and genistin in soybeans, soy nuts and soy powder were in the range of 4.6 to 18.2 and 200.6 to 968.1 mu g/g food, respectively. The values for soy milk and tofu (bean curd) were 1.9 to 13.9 and 94.8 to 137.7 mu g/g food, respectively. Levels of isoflavones in fermented soybean products, mise (bean paste) and natto (fermented soybeans), were 38.5 to 229.1 mu g/g food for genistein and 71.7 to 492.8 mu g/g food for genistin. Thus, the level or genistein in the fermented soybean products was higher than in soy beans and soybean products such as soy milk and tofu. From these observations, it is suggested that the beta-glycosyl bond of genistin is cleaved to produce genistein by microbes during fermentation to yield mise and natto. Soy sauce was also found to contain both isoflavones, but at levels lower than in mise and natto. On the basis of these data for average annual consumption of soybeans and related products, daily intake of genistein and genistin by the Japanese is calculated to be 1.5-4.1 and 6.3-8.3 mg/person, respectively. These levels are much higher than those for Americans or Western Europeans, whose mortality rates for breast, colon and prostate cancers are greater than the Japanese. (C) 1996 Elsevier Science Ltd.