Ubiquitous expression of mRFP1 in transgenic mice
Ubiquitous expression of mRFP1 in transgenic mice
复制标题
DOI：
10.1002/gene.20129
复制
发表时间：
2005-06-01
期刊：
影响因子：
1.5
通讯作者：
Wu, XH
中科院分区：
文献类型：
--
作者：
Zhu, HX;Wang, GX;Wu, XH
荧光蛋白为研究模型生物（如小鼠）提供了跟踪基因表达和细胞行为的有力手段。在新一代荧光蛋白标记物中，单体红色荧光蛋白mRFP 1特别有吸引力，因为其快速成熟并且对GFP和GFP衍生标记物的干扰最小。在这里，我们评估的效用mRFP 1作为转基因小鼠的标记。我们发现，高水平和普遍存在的mRFP 1表达不影响小鼠的发育，一般生理学，或生殖。在白化病背景的转基因小鼠中，mRFP 1表达可以在日光下用肉眼容易地检测到。mRFP 1信号的强度可用于区分纯合和杂合转基因小鼠。总之，这些特征使mRFP 1成为转基因研究中广泛应用的有吸引力的标记。（c）2005 Wiley-Liss，Inc.
Fluorescent proteins provide a powerful means to track gene expression and cellular behaviors in the study of model organisms such as mice. Among the new generation of fluorescent protein markers, the monomeric red fluorescent protein mRFP1 is particularly attractive because of its rapid maturation and minimal interference with GFP and GFP-derived markers. Here we evaluate the utility of mRFP1 as a marker in transgenic mice. We show that high level and ubiquitous expression of mRFP1 does not affect mouse development, general physiology, or reproduction. mRFP1 expression can be readily detected with unaided eyes under daylight in transgenic mice on the albino background. The intensity of mRFP1 signals can be used to distinguish homozygous and heterozygous transgenic mice. Together, these features make mRFP1 an attractive marker for broad applications in transgenic research. genesis (c) 2005 Wiley-Liss, Inc.