Radiation hybrid mapping of the zebrafish genome
Radiation hybrid mapping of the zebrafish genome
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DOI：
10.1073/pnas.96.17.9745
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发表时间：
1999-08-17
影响因子：
11.1
通讯作者：
Ekker, M
中科院分区：
文献类型：
--
作者：
Hukriede, NA;Joly, L;Ekker, M
斑马鱼是研究脊椎动物发育和疾病的优良遗传系统。为了给斑马鱼基因定位提供一种快速、可靠的工具，通过辐照斑马鱼AB9细胞与小鼠B78细胞融合，产生了一组辐射杂交体(RH)。斑马鱼序列在组成LN54面板的93个RH细胞系中的总保留率为22%。通过对具有849个简单序列长度多态标记、84个克隆基因和122个表达序列标签的LN54小组的鉴定，可以产生总大小为11,501 centirays的RH图谱。根据这个值，我们估计LN54RH面板的平均断点频率对应于1 centiray=148kbase。在RH图谱上放置一组235个无偏标记表明，目前为LN54面板生成的图谱覆盖了斑马鱼基因组的88%。RH图谱和减数分裂图谱中标记位置的比较表明，符合率为96%。利用LN54拼板定位表达序列标签和克隆基因是鉴定斑马鱼特定突变候选基因的一种有价值的方法。
The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation hybrids (RH) was produced by fusion of irradiated zebrafish AB9 cells with mouse B78 cells. The overall retention of zebrafish sequences in the 93 RH cell lines that constitute the LN54 panel is 22%. Characterization of the LN54 panel with 849 simple sequence length polymorphism markers, 84 cloned genes and 122 expressed sequence tags allowed the production of an RH map whose total size was 11,501 centiRays. From this value, we estimated the average breakpoint frequency of the LN54 RH panel to correspond to 1 centiRay = 148 kilobase. Placement of a group of 235 unbiased markers on the RH map suggests that the map generated for the LN54 panel, at present, covers 88% of the zebrafish genome. Comparison of marker positions in RH and meiotic maps indicated a 96% concordance. Mapping expressed sequence tags and cloned genes by using the LN54 panel should prove to be a valuable method for the identification of candidate genes for specific mutations in zebrafish.