Molecular cloning and tissue expression of canine Toll-like receptor 2 (TLR2)
Molecular cloning and tissue expression of canine Toll-like receptor 2 (TLR2)
复制标题
DOI：
10.1016/j.vetimm.2005.09.007
复制
发表时间：
2006-03-15
影响因子：
1.8
通讯作者：
Hasegawa, A
中科院分区：
文献类型：
--
作者：
Ishii, M;Hashimoto, M;Hasegawa, A
toll样受体2 （TLR2）在多种病原微生物的炎症反应中起重要作用。虽然一些研究表明人类和小鼠的TLR2基因多态性与感染或炎症反应有关，但犬的TLR2基因尚未得到研究。因此，本研究对犬toll样受体2基因cDNA全长进行了测序，并对犬TLR2 mRNA在不同组织中的表达进行了研究。犬TLR2克隆全长2552 bp，编码785个氨基酸。犬TLR2与人、小鼠、大鼠、牛、马和猪TLR2在核苷酸序列水平上的相似性分别为87.6、72.9、73.2、83.1、85.1和80.7%，在氨基酸序列水平上分别为84.6、68.7、68.5、78.8、82.9和79.0%。采用半定量逆转录聚合酶链反应（RT-PCR）方法，在犬血单个核细胞（BMCs）、淋巴结、肺、肝、脾、膀胱、胰腺、小肠、大肠和皮肤中检测到犬TLR2 mRNA。(c) 2005 Elsevier B.V.版权所有
Toll-like receptor 2 (TLR2) is involved in an important role on inflammatory reactions against various pathogenic microorganisms. Although some investigations suggested a relation between human and murine TLR2 gene polymorphisms and infections or inflammatory reactions, canine TLR2 gene has not been investigated. In the present study, therefore, the canine full-length of cDNA of Toll-like receptor 2 gene was sequenced and the expression of canine TLR2 mRNA was investigated in various tissues. Canine TLR2 clone was 2552 bp encoding 785 amino acids. The similarities of canine TLR2 with human, mouse, rat, cattle, horse and pig TLR2 were 87.6, 72.9, 73.2, 83.1, 85.1 and 80.7% at the nucleotide sequence level, and 84.6, 68.7, 68.5, 78.8, 82.9 and 79.0% at amino acid sequence level, respectively. By a semi quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis, mRNA of canine TLR2 was detected in blood mononuclear cells (BMCs), lymph node, lung, liver, spleen, bladder, pancreas, small intestine, large intestine and skin of the dogs. (c) 2005 Elsevier B.V. All rights reserved.