Bombyx mori nucleopolyhedrovirus Bm96 suppresses viral virulence in Bombyx mori larvae
Bombyx mori nucleopolyhedrovirus Bm96 suppresses viral virulence in Bombyx mori larvae
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家蚕核多角体病毒 Bm96 抑制家蚕幼虫的病毒毒力
DOI：
10.1016/j.jip.2020.107374
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发表时间：
2020
影响因子：
3.4
通讯作者：
Katsuma Susumu
中科院分区：
文献类型：
--
作者：
Hikida Hiroyuki;Kokusho Ryuhei;Matsuda-Imai Noriko;Katsuma Susumu
家蚕核型多角体病毒（BmNPV）是家蚕的一种严重病原。BmNPV在其128.4个内切酶对长的双链基因组中含有超过140个蛋白质编码基因。然而，许多BmNPV基因仍然是未知的。在此我们研究了BmNPVBm96在这两种B中的作用。桑椹细胞和幼虫。结果表明，Bm96主要在感染后期表达，在感染后24 h达到高峰，48 h后逐渐下降。桑椹细胞与野生型病毒相比，Bm96缺失型病毒在B.桑白皮。这些结果表明Bm96对BmNPV在B.桑白皮。此外，我们观察到感染Bm96缺失病毒的幼虫在感染后期表现出较低的运动活动，与感染野生型病毒的幼虫相比。
Bombyx mori nucleopolyhedrovirus(BmNPV) is a severe pathogen for the domestic silkworm,Bombyx mori. BmNPV harbors over 140 protein-coding genes in its 128.4 kilobase pair-long double-stranded genome. However, many BmNPV genes are still uncharacterized. Here we investigated the role of BmNPVBm96in bothB. moricultured cells and larvae. We found thatBm96is mainly expressed at the late stage of infection and accumulation of Bm96 protein peaks at 24 h post infection (hpi) and declines gradually at 48 hpi inB. moricultured cells. Compared with the wild-type viruses,Bm96-deletion viruses exhibited higher viral propagation and fast-killing phenotype inB. morilarvae. These results strongly suggest that Bm96 negatively regulates the propagation of BmNPV inB. morilarvae. Furthermore, we observed that larvae infected withBm96-deletion viruses showed lower locomotory activity at the late stage of infection compared with those infected with the wild-type viruses.