Effects of crustacean hyperglycemic hormone (CHH) on the transcript expression of carbohydrate metabolism-related enzyme genes in the kuruma prawn, Marsupenaeus japonicus
Effects of crustacean hyperglycemic hormone (CHH) on the transcript expression of carbohydrate metabolism-related enzyme genes in the kuruma prawn, Marsupenaeus japonicus
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DOI：
10.1016/j.ygcen.2011.03.019
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发表时间：
2011-06-01
影响因子：
2.7
通讯作者：
Nagasawa, Hiromichi
中科院分区：
文献类型：
--
作者：
Nagai, Chiaki;Nagata, Shinji;Nagasawa, Hiromichi
甲壳动物高血糖激素（CHH）是节肢动物特有的神经肽家族成员，在调节血淋巴葡萄糖水平、蜕皮、繁殖和应激反应中起着关键作用。虽然已经确定肝胰腺和肌肉是CHH调节高血糖活性的主要组织，但CHH调节碳水化合物代谢的分子机制仍不清楚。在这项研究中，我们分析了参与糖原代谢和糖原合成的酶的mRNA表达水平，以确定CHH如何调节血淋巴葡萄糖水平。我们首先从日本囊对虾（Marsupenaeus）中克隆了4种碳水化合物代谢相关酶的cDNA，分别是糖原磷酸化酶（MjGP）、糖原合成酶（MjGS）、果糖1，6-二磷酸酶（MjFBPase）和磷酸烯醇丙酮酸羧激酶（MjPEPCK）。RT-PCR分析表明，眼柄切除显著降低了肝胰腺中MjGP的表达，提高了MjGS的表达，但对肌肉中MjGP的表达无影响。考虑到各种眼柄因素，包括MIH，去除眼柄消融的事实，这些结果表明，眼柄消融后的代谢状态进行糖原积累在特定的组织相关的蜕皮。相比之下，MjFBPase和MjPEPCK转录水平没有显着改变眼柄切除，表明CHH和其他眼柄衍生的因素可能不会诱导胚胎发生。实时荧光定量PCR分析表明，暴露于重组CHH肝胰腺的MjGP和MjGS的表达水平显着改变，但没有MjFBPase和MjPEPCK。总的来说，这些结果表明，CHH参与肝胰腺糖原代谢。(C)2011 Elsevier Inc. All rights reserved.
Crustacean hyperglycemic hormone (CHH), a member of a neuropeptide family present only in arthropods, plays a pivotal role in the modulation of hemolymph glucose levels, molting, reproduction, and the stress response. Although it has been determined that hepatopancreas and muscle are the major tissues in which CHH regulates hyperglycemic activity, the molecular mechanism by which CHH regulates carbohydrate metabolism remains unclear. In this study, we analyzed the mRNA expression levels of enzymes involved in glycogen metabolism and gluconeogenesis in order to determine how CHH regulates hemolymph glucose levels. We first cloned cDNAs encoding four carbohydrate metabolism-related enzymes from the kuruma prawn, Marsupenaeus japonicus, glycogen phosphorylase (MjGP), glycogen synthase (MjGS), fructose 1,6-bisphosphatase (MjFBPase), and phosphoenolpyruvate carboxykinase (MjPEPCK). RT-PCR analysis showed that eyestalk ablation remarkably decreased MjGP and increased MjGS transcript levels in the hepatopancreas, but not in muscle. Considering the fact that various eyestalk factors, including MIH, are removed by eyestalk ablation, these results indicate that after eyestalk ablation the metabolic state proceeds towards glycogen accumulation in the specific tissues related to molting. In contrast, MjFBPase and MjPEPCK transcript levels were not significantly changed by eyestalk ablation, indicating that CHH and other eyestalk-derived factors might not induce gluconeogenesis. Quantitative real-time PCR analysis showed that exposure of hepatopancreas to recombinant CHH significantly changed the expression levels of MjGP and MjGS, but not MjFBPase and MjPEPCK. Collectively, these results indicate that CHH is involved in glycogen metabolism in hepatopancreas. (C) 2011 Elsevier Inc. All rights reserved.