Macrophage migration and invasion is regulated by MMP10 expression.
Macrophage migration and invasion is regulated by MMP10 expression.
复制标题
DOI：
10.1371/journal.pone.0063555
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gavrilovic J
中科院分区：
文献类型：
--
作者：
Murray MY;Birkland TP;Howe JD;Rowan AD;Fidock M;Parks WC;Gavrilovic J
This study was designed to identify metalloproteinase determinants of macrophage migration and led to the specific hypothesis that matrix metalloproteinase 10 (MMP10/stromelysin-2) facilitates macrophage migration. We first profiled expression of all MMPs in LPS-stimulated primary murine bone marrow-derived macrophages and Raw264.7 cells and found that MMP10 was stimulated early (3 h) and down-regulated later (24 h). Based on this pattern of expression, we speculated that MMP10 plays a role in macrophage responses, such as migration. Indeed, using time lapse microscopy, we found that RNAi silencing of MMP10 in primary macrophages resulted in markedly reduced migration, which was reversed with exogenous active MMP10 protein. Mmp10 −/− bone marrow-derived macrophages displayed significantly reduced migration over a two-dimensional fibronectin matrix. Invasion of primary wild-type macrophages into Matrigel supplemented with fibronectin was also markedly impaired in Mmp10 −/− cells. MMP10 expression in macrophages thus emerges as an important moderator of cell migration and invasion. These findings support the hypothesis that MMP10 promotes macrophage movement and may have implications in understanding the control of macrophages in several pathologies, including the abnormal wound healing response associated with pro-inflammatory conditions.
登录
查看更多内容
影响因子：
4.4
作者：
Ho, Hao H.;Antoniv, Taras T.;Ji, Jong-Dae;Ivashkiv, Lionel B.
通讯作者：
Ivashkiv, Lionel B.
影响因子：
3.7
作者：
Huang WC;Sala-Newby GB;Susana A;Johnson JL;Newby AC
通讯作者：
Newby AC
影响因子：
1.7
作者：
Bister, Ville;Maekitalo, Laura;Saarialho-Kere, Ulpu
通讯作者：
Saarialho-Kere, Ulpu
影响因子：
3.7
作者：
Bhavsar, Parag J.;Vigorito, Elena;Ridley, Anne J.
通讯作者：
Ridley, Anne J.
影响因子：
14.5
作者：
Bar-Or, A;Nuttall, RK;Yong, VW
通讯作者：
Yong, VW