Escherichia coli growth under modeled reduced gravity
Escherichia coli growth under modeled reduced gravity
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DOI：
10.1007/bf02870967
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发表时间：
2004-01-01
影响因子：
1.8
通讯作者：
Leff, LG
中科院分区：
文献类型：
--
作者：
Baker, PW;Meyer, ML;Leff, LG
细菌表现出不同的反应，模拟减少重力，可以模拟倾斜旋转。当大肠杆菌是不同的旋转速度在倾斜旋转，显着差异之间的建模减少重力和正常重力控制，只有在较高的速度（30-50 rpm）。当E.大肠杆菌在基本培养基（40 rpm）中生长，细胞大小不受模拟重力降低的影响，细胞数量几乎没有差异。然而，在较高的营养条件下（即。稀释的营养肉汤），与正常重力对照相比，在降低的重力下总细胞数更高并且细胞更小。总的来说，模拟减少重力的反应随营养条件而变化;较大的表面体积比可能有助于补偿模拟减少重力下细胞周围的营养耗尽区。
Bacteria exhibit varying responses to modeled reduced gravity that can be simulated by clino-rotation. When Escherichia coli was different rotation speeds during clino-rotation, significant differences between modeled reduced gravity and normal gravity controls were observed only at higher speeds (30-50 rpm). There was no apparent affect of removing samples on the results obtained When E. coli was grown in minimal medium (at 40 rpm), cell size was not affected by modeled reduced gravity and there were few differences in cell numbers. However, in higher nutrient conditions (i.e.. dilute nutrient broth), total cell numbers were higher and cells were smaller under reduced gravity compared to normal gravity controls. Overall, the responses to modeled reduced gravity varied with nutrient conditions; larger surface to volume ratios may help compensate for the zone of nutrient depletion around the cells under modeled reduced gravity.