Comparison of cardiolipins from Drosophila strains with mutations in putative remodeling enzymes
Comparison of cardiolipins from Drosophila strains with mutations in putative remodeling enzymes
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DOI：
10.1016/j.chemphyslip.2012.03.001
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Neubert, Thomas A.
中科院分区：
文献类型：
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作者：
Schlame, Michael;Blais, Steven;Neubert, Thomas A.
心磷脂是一种二聚体磷脂，具有特征性酰基组成，由从头合成后脂肪酸重塑产生。已经提出几种酶参与心磷脂的酰基重塑。为了比较这些酶的效果，我们确定了特定酶缺失的果蝇品系中的心磷脂分子种类的模式，使用MALDI-TOF质谱与内标。我们建立了心磷脂定量方法的线性范围，确定了几个心磷脂标准品的相对信号强度，并证明了令人满意的信噪比心磷脂光谱从一个单一的苍蝇。我们的数据表明在果蝇的生命周期中的心磷脂成分的变化。使用向量代数比较心磷脂谱显示，他法津的失活对心磷脂的分子组成有很大影响，钙非依赖性磷脂酶A（2）的失活影响很小，而酰基辅酶A：溶血心磷脂酰基转移酶和三功能酶的失活不影响心磷脂的组成。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Cardiolipin is a dimeric phospholipid with a characteristic acyl composition that is generated by fatty acid remodeling after de novo synthesis. Several enzymes have been proposed to participate in acyl remodeling of cardiolipin. In order to compare the effect of these enzymes, we determined the pattern of cardiolipin molecular species in Drosophila strains with specific enzyme deletions, using MALDI-TOF mass spectrometry with internal standards. We established the linear range of the method for cardiolipin quantification, determined the relative signal intensities of several cardiolipin standards, and demonstrated satisfying signal-to-noise ratios in cardiolipin spectra from a single fly. Our data demonstrate changes in the cardiolipin composition during the Drosophila life cycle. Comparison of cardiolipin spectra, using vector algebra, showed that inactivation of tafazzin had a large effect on the molecular composition of cardiolipin, inactivation of calcium-independent phospholipase A(2) had a small effect, whereas inactivation of acyl-CoA:lysocardiolipin-acyltransferase and of the trifunctional enzyme did not affect the cardiolipin composition. (C) 2012 Elsevier Ireland Ltd. All rights reserved.