DETERMINATION OF CYSTEINESULFINATE, HYPOTAURINE AND TAURINE IN PHYSIOLOGICAL SAMPLES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
DETERMINATION OF CYSTEINESULFINATE, HYPOTAURINE AND TAURINE IN PHYSIOLOGICAL SAMPLES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
复制标题
DOI：
10.1016/s0378-4347(00)84599-5
复制
发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
STIPANUK, MH
中科院分区：
文献类型：
--
作者：
HIRSCHBERGER, LL;DELAROSA, J;STIPANUK, MH
利用反相高效液相色谱法在沃茨Nova-Pak C18柱上分离半胱氨酸的关键代谢产物半胱氨酸亚磺酸盐、亚牛磺酸和牛磺酸，并将其与生物样品中的其他近距离洗脱氨基酸分离。进样前，用邻苯二甲醛-2-巯基乙醇衍生样品。洗脱系统由100 mM磷酸钾缓冲液（pH 7.0）和3%（v/v）四氢呋喃组成，初始等度相为1.2%乙腈，梯度为1.2 - 12.8%乙腈。该方法适用于测定分离细胞半胱氨酸代谢产物的生成量以及血浆和组织提取物的分析。低水平的亚牛磺酸在大鼠组织中很容易用这种方法测量，并在这里首次报道。
Cysteinesulfinate, hypotaurine and taurine, which are key metabolites of cysteine, can be separated from each other and other closely eluting amino acids in biological samples by reversed-phase high-performance liquid chromatography on a Waters Nova-Pak C18 column. Samples were derivatized with o-phthalaldehyde-2-mercaptoethanol prior to injection. The elution system consisted of 100 mM potassium phosphate buffer, pH 7.0, with 3% (v/v) tetrahydrofuran with an initial isocratic phase at 1.2% acetonitrile and a gradient from 1.2 to 12.8% acetonitrile. This method is suitable for measurement of the production of metabolites from cysteine by isolated cells and for anlysis of plasma and tissue extracts. Low levels of hypotaurine in rat tissues were easily measured with this method and are reported here for the first time.