Synthesis of Water‐Soluble Dendritic Conjugated Polymers for Fluorescent DNA Assays
Synthesis of Water‐Soluble Dendritic Conjugated Polymers for Fluorescent DNA Assays
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DOI：
10.1002/marc.200600495
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发表时间：
2006-10
影响因子：
4.6
通讯作者：
Minghui Yu;Yanli Tang;F. He;Shu Wang;Da-Gui Zheng;Yuliang Li;Daoben Zhu
中科院分区：
文献类型：
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作者：
Minghui Yu;Yanli Tang;F. He;Shu Wang;Da-Gui Zheng;Yuliang Li;Daoben Zhu
阳离子水溶性树枝状聚芴（PEP-G 0-2）是由大分子单体（逐代）合成的。PEP-G 0-2的电荷密度可以通过静电相互作用控制其与DNA的距离。具有更高代次的树枝状侧链的PFP-G 2具有更高的电荷密度，并产生有效的荧光共振能量转移（FRET）至标记在DNA末端的信号荧光素。这些新的共轭聚合物在四氢呋喃/H2O混合溶剂中形成的聚集体较少，产生的FRET效率更高。通过使用高度树枝状的共轭聚合物，提高了DNA传感器的灵敏度。
Cationic water-soluble dendritic poly(fluorene)s with positively charged amines on the exterior (PEP-G 0-2 ) can be prepared by a macromonomer (generation-by-generation) approach. The charge densities of PEP-G 0-2 can control their distances to DNA by electrostatic interactions. The PFP-G 2 with higher generation of dendritic side chains possesses a higher charge density, and gives rise to efficient fluorescence resonance energy transfer (FRET) to a signaling fluorescein labeled at the terminus of DNA. These new conjugated polymers form less aggregates and yield more efficient FRET in a tetrahydrofuran/H 2 O mixed solvent as compared to in pure water. The sensitivity of the DNA sensor is improved by utilizing highly dendritic conjugated polymers.