Effect of the lactic acid bacterium Streptococcus thermophilus on ceramide levels in human keratinocytes in vitro and stratum corneum in vivo
Effect of the lactic acid bacterium Streptococcus thermophilus on ceramide levels in human keratinocytes in vitro and stratum corneum in vivo
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DOI：
10.1046/j.1523-1747.1999.00633.x
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发表时间：
1999-07-01
影响因子：
6.5
通讯作者：
Cifone, MG
中科院分区：
文献类型：
--
作者：
Di Marzio, L;Cinque, B;Cifone, MG
研究了嗜热链球菌对体外培养的人角质形成细胞或体内角质层神经酰胺水平的影响。在体外，嗜热链球菌以时间依赖性的方式增强角质形成细胞中神经酰胺的水平。嗜热链球菌中存在高水平的中性谷胱甘肽敏感性鞘磷脂酶可能是观察到的神经酰胺增加的原因。在17名健康志愿者前臂皮肤中应用含有超声处理的嗜热链球菌的基础霜7 d也导致皮肤神经酰胺量的显著和相关增加，这可能是由于通过细菌中性鞘磷脂酶水解鞘磷脂。实际上，用含有纯化的细菌中性鞘磷脂酶的基础乳膏获得了类似的结果。此外，通过谷胱甘肽抑制细菌中性鞘磷脂酶活性阻断了治疗后观察到的皮肤神经酰胺增加。局部应用超声波处理的嗜热链球菌制剂，导致角质层神经酰胺水平增加，从而可以改善脂质屏障和更有效地抵抗干燥症。
The effects of Streptococcus thermophilus on ceramide levels either in vitvo on cultured human keratinocytes or in vivo on stratum corneum, have been investigated. In vitro, Streptococcus thermophilus enhanced the levels of ceramides in keratinocytes in a time-dependent way. The presence of high levels of neutral, glutathione-sensitive, sphingomyelinase in Streptococcus thermophilus could be responsible for the observed ceramide increase. The application of a base cream containing sonicated Streptococcus thermophilus in the forearm skin of 17 healthy volunteers for 7 d also led to a significant and relevant increase of skin ceramide amounts, which could be due to the sphingomyelin hydrolysis through bacterial neutral sphingomyelinase. Indeed, similar results were obtained with a base cream containing purified bacterial neutral sphingomyelinase. In addition, the inhibition of bacterial neutral sphingomyelinase activity through glutathione blocked the skin ceramide increase observed after the treatment. The topical application of a sonicated Streptococcus thermophilus preparation, leading to increased stratum corneum ceramide levels, could thus result in the improvement of lipid barrier and a more effective resistance against xerosis.