Juvenile hormone and allatostatins in the German cockroach embryo
Juvenile hormone and allatostatins in the German cockroach embryo
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DOI：
10.1016/j.ibmb.2010.06.006
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发表时间：
2010-09-01
影响因子：
3.8
通讯作者：
Belles, Xavier
中科院分区：
文献类型：
--
作者：
Maestro, Jose L.;Pascual, Nuria;Belles, Xavier
测定了德国小蠊胚胎保幼激素III（JH）、FGL酰胺类allatostatin肽（ASTs）、ASTs前体（preproAST）mRNA和法尼基酸甲酯环氧酶（CYP 15 A1）mRNA的水平。JH在背侧闭合后开始上升，在胚胎发生的60%至80%之间达到最大水平，随后下降至不可检测的水平。AST在胚胎发生的前三分之二期间水平较低，此后增加，并保持高水平，直到孵化。前-proAST mRNA在产卵后的两天内显示出相当高的水平，因此表现为母体转录物，然后水平变得非常低，直到胚胎发育中期，然后增加，在胚胎发育结束时达到峰值。在约25%的胚胎发生中检测到CYP 15 A1转录物，并且该水平倾向于通过胚胎发生增加，尽管研究天数之间的差异无统计学显著性。在胚胎发育末期，JH和AST的表达模式相反，沿着晚期胚胎咽侧体中AST的表达，提示JH的下降是由于AST的升高所致。此外，JH浓度和CYP 15 A1 mRNA水平的不相关模式表明，CYP 15 A1表达不调节JH的生产。(C)2010爱思唯尔有限公司保留所有权利。
Levels of juvenile hormone III (JH), FGLamide allatostatin peptides (ASTs), ASTs precursor (preproAST) mRNA and methyl farnesoate epoxidase (CYP15A1) mRNA were measured in embryos of the cockroach Blattella germanica. JH starts to rise just after dorsal closure, reaches maximal levels between 60% and 80% of embryogenesis, and decrease subsequently to undetectable levels. ASTs show low levels during the first two thirds of embryogenesis, increase thereafter and maintain high levels until hatching. Pre-proAST mRNA shows quite high levels during the two days following oviposition, thus behaving as a maternal transcript, the levels then become very low until mid embryogenesis, and increase afterwards, peaking towards the end of embryo development. CYP15A1 transcripts were detected around 25% embryogenesis and the levels tended to increase through embryogenesis, although differences amongst the days studied were not statistically significant. The opposite patterns of JH and AST towards the end of embryo development, along with the detection of AST immunoreactivity in corpora allata from late embryos, suggest that JH decline is caused by the increase of AST. Moreover, the uncorrelated patterns of JH concentration and CYP15A1 mRNA levels suggest that CYP15A1 expression does not modulate JH production. (C) 2010 Elsevier Ltd. All rights reserved.