REFERENCE SAMPLE MICROSPHERE METHOD - CARDIAC-OUTPUT AND BLOOD FLOWS IN CONSCIOUS RAT
REFERENCE SAMPLE MICROSPHERE METHOD - CARDIAC-OUTPUT AND BLOOD FLOWS IN CONSCIOUS RAT
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DOI：
10.1152/ajpheart.1980.239.4.h443
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发表时间：
1980-01-01
影响因子：
--
通讯作者：
FROHLICH, ED
中科院分区：
文献类型：
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作者：
ISHISE, S;PEGRAM, BL;FROHLICH, ED
通过以下方法完成了大鼠中参比样品方法（RSM）的验证：在乙醚麻醉开胸Wistar大鼠中通过RSM和电磁流量法同时测量心输出量（CO）;以及在3次连续注射微球后在清醒大鼠中测定全身和局部血流动力学重现性。确定了几种CO状态下的最佳采样时间和采样速率。显然，200个或更多的微球包含在参考样品中的结果在CO的准确测定。几乎100%的微球被捕获的组织在20秒的微球注射停止。血流动力学稳态仅在高采样率（1.211 ml/min）下的低CO条件下受到影响。在清醒的动物中重复注射三次微球不会改变全身血流动力学。除肝、胃、脾和皮肤（第3次应用）外，所有器官的局部血流分布重现性良好。RSM显然是可靠的，提供了合理的准确性和再现性的全身和局部血流动力学的大鼠。
Validation of the reference sample method (RSM) in the rat was accomplished by the following: simultaneous measurement of cardiac output (CO) by the RSM and electromagnetic flowmetry method in ether-anesthetized open-chest Wistar rats; and determination of systemic and regional hemodynamic reproducibility in conscious rats following 3 serial injections of microspheres. The optimal sampling time and sampling rate were determined in several CO states. Apparently, 200 or more microspheres contained in the reference sample result in an accurate determination of CO. Almost 100% of the microspheres are trapped by tissues with in 20 s of cessation of the microsphere injection. The hemodynamic steady state is affected only under low CO conditions at a high sampling rate (1.211 ml/min). Three repeated injections of microspheres in conscious animals do not alter systemic hemodynamics. The reproducibility of regional blood flow distribution is good in all organs except the liver, stomach, spleen and skin (3rd application). The RSM evidently is reliable, providing reasonable accuracy and reproducibility of systemic and regional hemodynamics in the rat.