Isolation of a Leptothrix Strain, OUMS1, from Ocherous Deposits in Groundwater
Isolation of a Leptothrix Strain, OUMS1, from Ocherous Deposits in Groundwater
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DOI：
10.1007/s00284-011-9957-6
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发表时间：
2011-08-01
影响因子：
2.6
通讯作者：
Takada, Jun
中科院分区：
文献类型：
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作者：
Sawayama, Michinori;Suzuki, Tomoko;Takada, Jun
水生环境中的细毛虫产生形状独特的中空微管，由水生无机和细菌衍生的有机杂交物组成。我们小组将这种生物来源的氧化铁称为“生物来源氧化铁(BIOX)”。大多数工业氧化铁的人工合成需要大量能源，而且成本高昂，而从自然环境中合成BIOX是能源和成本效益高的。如果开发出有效和高效的应用，BIOX微管可能被用作催化剂、吸附剂和颜料等的新型工业功能资源。为此，必须建立一个可重现的系统来调节细菌及其BIOX生产力，以根据工业需求提供足够量的BIOX。然而，目前对BIOX微管形成的细菌种类和形成机制知之甚少。在本研究中，一种新的细毛藻Leptothrix sp.通过对不同培养基及培养条件的试验，从地下水沉淀物中成功分离到一株菌株OUMS1。对已知的霍乱弧菌SP-6菌株的形态、生理特性和元素组成进行了比较，并比较了两株菌株之间的差异。OUMS1的成功分离使我们建立了一个基本的系统，积累了细毛虫的生物学知识，促进了对微管形成机制的理解。对OUMS1相关微结构的更多地球化学研究有望为研究细菌来源的氧化铁的广泛工业应用提供一种有吸引力的方法。
Leptothrix species in aquatic environments produce uniquely shaped hollow microtubules composed of aquatic inorganic and bacterium-derived organic hybrids. Our group termed this biologically derived iron oxide as "biogenous iron oxide (BIOX)". The artificial synthesis of most industrial iron oxides requires massive energy and is costly while BIOX from natural environments is energy and cost effective. The BIOX microtubules could potentially be used as novel industrial functional resources for catalysts, adsorbents and pigments, among others if effective and efficient applications are developed. For these purposes, a reproducible system to regulate bacteria and their BIOX productivity must be established to supply a sufficient amount of BIOX upon industrial demand. However, the bacterial species and the mechanism of BIOX microtubule formation are currently poorly understood. In this study, a novel Leptothrix sp. strain designated OUMS1 was successfully isolated from ocherous deposits in groundwater by testing various culture media and conditions. Morphological and physiological characters and elemental composition were compared with those of the known strain L. cholodnii SP-6 and the differences between these two strains were shown. The successful isolation of OUMS1 led us to establish a basic system to accumulate biological knowledge of Leptothrix and to promote the understanding of the mechanism of microtubule formation. Additional geochemical studies of the OUMS1-related microstructures are expected provide an attractive approach to study the broad industrial application of bacteria-derived iron oxides.