DNA determinants of promoter selectivity in Escherichia coli.
DNA determinants of promoter selectivity in Escherichia coli.
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大肠杆菌启动子选择性的 DNA 决定因素。
DOI：
10.1101/sqb.1983.047.01.057
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
Susskind,MM
中科院分区：
文献类型：
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作者：
McClure,WR;Hawley,DK;Youderian,P;Susskind,MM
DNA特定区域的转录速率似乎主要受到RNA聚合酶利用多种特定DNA序列（启动子）启动转录的频率的限制。在体内观察到的RNA起始频率的1000倍范围被认为反映了这种启动子序列的多样性；因此，对大肠杆菌转录选择性的研究主要集中在启动子结构与起始频率的关系上。那么，启动子结构是如何决定起始频率的呢？Zillig和他的同事（Walter et al. 1967）提出了一个简单的启动功能机制。所涉及的两个步骤示意图如下：
The rates of transcription of particular regions of DNA appear to be limited primarily by the frequencies with which RNA polymerase can utilize a diversity of specific DNA sequences (promoters) to initiate transcription. The 1000-fold range of RNA initiation frequencies observed in vivo is thought to reflect this diversity of promoter sequences; consequently, the investigation of transcriptional selectivity in Escherichia coli has focused largely on the relationship of promoter structure to initiation frequency. How, then, does promoter structure determine initiation frequency? A simple functional mechanism for initiation was suggested by Zillig and co-workers (Walter et al. 1967). The two steps involved are shown schematically as follows: