FOCAL ADHESION KINASE IN RAT CENTRAL-NERVOUS-SYSTEM
FOCAL ADHESION KINASE IN RAT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1111/j.1460-9568.1995.tb00700.x
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发表时间：
1995-08-01
影响因子：
3.4
通讯作者：
GIRAULT, JA
中科院分区：
文献类型：
--
作者：
BURGAYA, F;MENEGON, A;GIRAULT, JA
粘着斑激酶（Focal adhesion kinase，pp（125FAK），FAK）是一种125 kDa的非受体酪氨酸激酶，在各种细胞类型的粘着斑中富集，在粘着斑中，它被认为是通过与细胞外基质接触而触发的信号的抑制剂。我们研究了粘着斑激酶（FAK）在大鼠中枢神经系统的表达和定位。免疫印迹，免疫组织化学和原位杂交显示FAK在成人大脑的所有区域的存在，并证明其富集在特定的神经元群体的大脑和小脑皮质，以及在海马。在发育过程中，FAK蛋白水平在出生前后大脑皮层和尾壳核中最高，成年后下降。原位杂交结果显示，在胚胎生长的最后阶段，FAK mRNA在心室萌发层和外层富集。在原代培养中，FAK免疫反应性定位于星形胶质细胞中的粘着斑，而在发育中的神经元中，生长锥和胞体中的水平最高。在生长锥，FAK免疫反应共定位与肌动蛋白丝。在成熟的神经元中，FAK似乎分布在整个细胞质中，在任何细胞隔室中都没有富集。我们的研究结果表明，存在高水平的FAK在大鼠中枢神经系统，最大的发展过程中，但在成人持久。它在生长锥中的富集表明，它可能在神经突生长中发挥作用，以及在成人的可塑性。
Focal adhesion kinase (pp(125FAK), FAK) is a 125 kDa non-receptor tyrosine kinase enriched in focal adhesions of various cell types, where it is thought to transduce signals triggered by contact with the extracellular matrix. We have studied the expression and localization of FAK in rat CNS. Immunoblotting, immunohistochemistry and in situ hybridization revealed the presence of FAK in all regions of the adult brain and demonstrated its enrichment in specific neuronal populations of the cerebral and cerebellar cortex, as well as in the hippocampus. During development, FAK protein levels were highest around birth in cerebral cortex and caudate putamen and decreased in the adult. In situ hybridization revealed enrichment of FAK mRNA in the ventricular germinative and external layers during the last period of embryonic growth. In primary cultures FAK immunoreactivity was localized in focal adhesions in astrocytes, whereas in developing neurons the highest levels were found in growth cones and perikarya. In the growth cone, FAK immunoreactivity colocalized with actin filaments. In mature neurons FAK appeared to be distributed in the whole cytoplasm, with no enrichment in any cellular compartment. Our results demonstrate the presence of high levels of FAK in rat CNS, maximal during development but persistent in the adult. Its enrichment in growth cones suggests that it may play a role in neurite outgrowth, as well as in plasticity in the adult.