BRU1 maintains configuration of the euchromatic subchromosomal domain in the nucleus of Arabidopsis.
BRU1 maintains configuration of the euchromatic subchromosomal domain in the nucleus of Arabidopsis.
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BRU1 维持拟南芥细胞核中常染色质亚染色体结构域的构型。
DOI：
10.1007/s11105-013-0596-x
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
S.
中科院分区：
文献类型：
--
作者：
Ohno;Y;Nishimura;T.;Hattori;T.;and Takeda;S.
染色质状态与基因调控密不可分。在酵母和动物中，染色质状态也与细胞核中染色体结构域的结构和空间定位模式相关。然而，在植物中，除了异色结构域外，对这些与基因调控相关的结构域的动力学知之甚少。我们之前已经报道了拟南芥染色体的几个染色质区域，其中基因被bru1的缺陷优先上调，bru1是一种参与DNA损伤反应和表观遗传基因调控的核因子。在这项研究中，我们提出了其中一个亚染色体区域SCR1的细胞遗传学特征。在野生型叶细胞的细胞核中，174 kb的SCR1区域在核仁组织区4 （NOR4）附近适度浓缩。在突变体中，SCR1的局限定位模式被随机打乱。相比之下，bru1缺陷不影响NOR4和另一个149-kb的常染色质区域的定位模式，该区域的基因活性在bru1中没有改变。在叶片、下胚轴和未分化愈伤组织中，细胞核中SCR1的限制程度不同，但不明显。这些结果表明，BRU1在维持常染色质亚染色体结构域的结构中起作用，这是控制基因活性的潜在决定因素。
Chromatin states are inseparably associated with regulation of genes. In yeast and animals, chromatin states also correlate with the configuration and spatial localization pattern of chromosomal domains in the nucleus. In plants, however, the dynamics of such domains associated with gene regulation is poorly understood except for heterochromatic domains. We have previously reported several euchromatic regions of Arabidopsis chromosomes where genes are preferentially upregulated by a defect in BRU1—a nuclear factor involved in DNA damage responses and epigenetic gene regulation. In this study, we present a cytogenetic characterization of one of these subchromosomal regions, SCR1. In nuclei of wild-type leaf cells, the 174-kb SCR1 region was moderately condensed near nucleolus organizing region 4 (NOR4). Inbru1mutants, the confined localization pattern of SCR1 was stochastically disrupted. In contrast,bru1defects did not affect the localization patterns of NOR4 and another 149-kb euchromatic region in which gene activity was not altered inbru1. The degree of confinement of SCR1 in the nucleus varied between leaves, hypocotyls, and undifferentiated calli, but not drastically. These results suggest that BRU1 plays a role in maintaining the configuration of a euchromatic subchromosomal domain that is a potential determinant in the control of gene activity.