Structure of the genomic sequence comprising the immunoglobulin heavy constant (IGHC) genes from Sus scrofa
Structure of the genomic sequence comprising the immunoglobulin heavy constant (IGHC) genes from Sus scrofa
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DOI：
10.1016/j.molimm.2012.05.010
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发表时间：
2012-10-01
影响因子：
3.6
通讯作者：
Uenishi, Hirohide
中科院分区：
文献类型：
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作者：
Eguchi-Ogawa, Tomoko;Toki, Daisuke;Uenishi, Hirohide
免疫球蛋白重链（IGH）恒定基因（IGHC）的数量在哺乳动物中变化。为了注释猪IGHC基因，我们对来自单个猪单倍型的整个含有IGHC的基因组区域进行测序。得到的连续序列包括在5'IGH多样性（D）基因簇和3' TMEM121中，其位于人基因组中IGHC簇的侧翼，表明我们已经获得了含有猪IGHC的整个基因组区域。该区域长约190 kb，与其他哺乳动物的区域一致。猪IGHC基因簇包括IGHM、IGHD、6个IGHG基因、IGHE和IGHA共10个基因。猪IGHG基因在IGHD和IGHE之间形成一个簇，IGHG 3被认为是最古老的IGHG基因，位于IGHG簇的开始。此外，猪序列含有两个IGHG 5和两个IGHG 6基因，但不含IgG 2和IgG 4的IGHG基因，表明IGHG簇内的灵活性。我们还记录了IGHC基因的开关区域的结构差异，这可能对它们的转录很重要。该单倍型可作为今后研究其他单倍型和猪免疫球蛋白（IG）同种型的功能分析的参考。(C)2012爱思唯尔有限公司保留所有权利。
The number of immunoglobulin heavy chain (IGH) constant genes (IGHC) varies among mammals. To annotate the porcine IGHC genes, we sequenced the entire IGHC-containing genomic region from a single porcine haplotype. The resulting contiguous sequence included in 5' the IGH diversity (D) gene cluster and in 3' TMEM121, which flank the IGHC cluster in the human genome, suggesting that we had obtained the entire genomic region containing porcine IGHC. This region was about 190-kb long, in good agreement with those of other mammals. The porcine IGHC cluster contained 10 genes, IGHM, IGHD, six IGHG genes, IGHE and IGHA. The porcine IGHG genes formed a cluster between IGHD and IGHE, with IGHG3 considered as the most ancient IGHG gene, located at the beginning of the IGHG cluster. Furthermore, the porcine sequence contained two IGHG5 and two IGHG6 genes, but no IGHG genes for IgG2 and IgG4, suggesting flexibility within the IGHG cluster. We also recorded structural differences in the switch regions of the IGHC genes that may be important in their transcription. This haplotype can serve as a reference for future studies on other haplotypes and for functional analysis of porcine immunoglobulin (IG) isotypes. (C) 2012 Elsevier Ltd. All rights reserved.