Construction of a BAC library of Rosa rugosa Thunb. and assembly of a contig spanning Rdr1, a gene that confers resistance to blackspot
Construction of a BAC library of Rosa rugosa Thunb. and assembly of a contig spanning Rdr1, a gene that confers resistance to blackspot
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DOI：
10.1007/s00438-002-0784-0
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发表时间：
2003-02-01
影响因子：
3.1
通讯作者：
Debener, T
中科院分区：
文献类型：
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作者：
Kaufmann, H;Mattiesch, L;Debener, T
以玫瑰为材料构建了BAC文库，为玫瑰基因的定位和定位克隆提供了通用工具。27，264个克隆的文库含有5.2个基因组当量。该文库用于组装跨越Rdr 1（赋予对黑斑病抗性的基因座）的BAC克隆的重叠群。为此，使用816个AFLP引物组合通过混合分离分析实现了目标基因座的精细定位。Rdr 1周围的靶区域包含约400 kb，并被最少6个BAC克隆覆盖。此外，在重叠群上检测到TIR-NBS-LRR家族的至少五个抗性基因类似物表明在Rdr 1周围存在抗性基因簇。这些结果将不仅允许Rdr 1的分离和鉴定在不久的将来，但也提供了工具，在玫瑰中的其他园艺感兴趣的基因的物理定位和定位克隆。
A BAC library to serve as a general tool for the physical mapping and positional cloning of rose genes has been constructed from Rosa rugosa DNA. With 27,264 clones the library contains 5.2 genome equivalents. The library was used to assemble a contig of BAC clones spanning Rdr1, a locus that confers resistance to blackspot. For this purpose fine-scale mapping of the target locus was achieved by bulked segregant analysis using 816 AFLP primer combinations. The target region around Rdr1 comprises about 400 kb and is covered by a minimum of six BAC clones. Furthermore, the detection of at least five resistance gene analogs of the TIR-NBS-LRR family on the contig indicates the presence of a cluster of resistance genes around Rdr1. These results will not only allow the isolation and identification of Rdr1 in the near future, but also provide the tools for the physical mapping and positional cloning of other horticulturally interesting genes in roses.