A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer
A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer
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DOI：
10.1002/bio.1352
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发表时间：
2012-09-01
期刊：
影响因子：
2.9
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
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作者：
Arakawa, Takahiro;Ando, Eri;Mitsubayashi, Kohji
采用高纯度鲁米诺溶液和化学发光（CL）增强剂的辣根过氧化物-鲁米诺-过氧化氢体系，建立了二维气态乙醇可视化体系。该系统通过鲁米诺反应测量乙醇浓度作为CL的强度。当将气态乙醇注射到酶固定膜上时，CL被释放，该膜被用作二维气体可视化的屏幕。底物上CL的平均强度与标准乙醇气体浓度呈线性相关。将这些结果与可视化系统中CCD相机记录图像的CL强度进行了比较。该系统不仅可用于气体组分的空间分析，还可用于实时的时间分析。采用高纯度钠盐HG溶液（L-HG）代替标准鲁米诺溶液，并用伊红Y （EY）溶液增强体系的CL强度。在检测限为3？L-HG溶液和EY最佳浓度下的ppm。版权所有：John Wiley & Sons， Ltd。
A two-dimensional gaseous ethanol visualization system has been developed and demonstrated using a horseradish peroxidaseluminolhydrogen peroxide system with high-purity luminol solution and a chemiluminescence (CL) enhancer. This system measures ethanol concentrations as intensities of CL via the luminol reaction. CL was emitted when the gaseous ethanol was injected onto an enzyme-immobilized membrane, which was employed as a screen for two-dimensional gas visualization. The average intensity of CL on the substrate was linearly related to the concentration of standard ethanol gas. These results were compared with the CL intensity of the CCD camera recording image in the visualization system. This system is available for gas components not only for spatial but also for temporal analysis in real time. A high-purity sodium salt HG solution (L-HG) instead of standard luminol solution and an enhancer, eosin Y (EY) solution, were adapted for improvement of CL intensity of the system. The visualization of gaseous ethanol was achieved at a detection limit of 3?ppm at optimized concentrations of L-HG solution and EY. Copyright (c) 2011 John Wiley & Sons, Ltd.