Control of Fusarium wilt in melon by the fungal endophyte, Cadophora sp.
Control of Fusarium wilt in melon by the fungal endophyte, Cadophora sp.
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DOI：
10.1007/s10658-014-0389-6
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发表时间：
2014-06-01
影响因子：
1.8
通讯作者：
Narisawa, Kazuhiko
中科院分区：
文献类型：
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作者：
Khastini, Rida O.;Ogawara, Takashi;Narisawa, Kazuhiko
两种土传真菌内生菌几乎完全抑制了后接种和尖孢镰刀菌f。当接种到培养皿中的无菌饲养的甜瓜幼苗时，甜瓜炭疽病菌（sp. melonis）感染甜瓜。根据ITS 1-5.8S rDNA-ITS 2序列和形态特征，从生长在加拿大阿尔伯塔和不列颠哥伦比亚省不同森林土壤混合土壤中的诱饵植物大白菜根部分离到一株刚毛藻属（Cadophora sp.）菌丝沿着根表面生长，并定殖于皮层的根细胞，减少镰刀菌病原体进入相邻细胞。还将用刚毛藻属物种预接种的甜瓜幼苗生长在用不同N源、硝酸盐或氨基酸亮氨酸和缬氨酸以及葡萄糖（最终C：N比= 10：1）改良的土壤中。4周后，将这些幼苗移植到田间，并评估疾病症状。只有经缬氨酸处理的内生菌接种苗能有效地抑制枯萎病在两个小区中的发展，使病害症状发展减少了43%和62%。
Two soil-borne fungal endophytes almost completely suppressed the effects of a post-inoculated and virulent strain of Fusarium oxysporum f. sp. melonis when inoculated to axenically reared melon seedlings in Petri dishes. They were identified as Cadophora sp. on the basis of ITS 1-5.8S rDNA-ITS 2 sequences and morphological characters and obtained from the roots of Chinese cabbage grown as bait plants in a mixed soil made up of samples from different forest soils from Alberta and British Columbia, Canada. Hyphae of Cadophora sp. grew along the surface of the root and colonized root cells of the cortex and reduced the ingress of the Fusarium pathogen into adjacent cells. Melon seedlings pre-inoculated with Cadophora sp. were also grown in soil amended with the different N sources, nitrate or the amino acids leucine and valine, and glucose (final C:N ratio = 10:1). After 4 weeks, these seedlings were transplanted into the field and disease symptoms were assessed. Only the endophyte-inoculated seedlings treated with valine could effectively inhibit the development of Fusarium wilt in two plots and reduced disease symptom development by 43 and 62 %.