Direct Analysis of Methylated Trivalent Arsenicals in Mouse Liver by Hydride Generation-Cryotrapping-Atomic Absorption Spectrometry
Direct Analysis of Methylated Trivalent Arsenicals in Mouse Liver by Hydride Generation-Cryotrapping-Atomic Absorption Spectrometry
复制标题
DOI：
10.1021/tx200060c
复制
发表时间：
2011-04-01
影响因子：
4.1
通讯作者：
Styblo, Miroslav
中科院分区：
文献类型：
--
作者：
Currier, Jenna M.;Svoboda, Milan;Styblo, Miroslav
越来越多的证据表明，无机砷（iAs）的甲基化三价代谢物，甲基拉森石（MAsIII）和二甲基拉森石（DMAsIII），有助于iAs暴露的不良影响。然而，缺乏合适的方法阻碍了复杂生物基质中MAsIII和DMAsIII的定量分析。在这里，我们发现氢化物发生-低温-原子吸收光谱法可以定量暴露于iAs的小鼠肝脏中的MAsIII和DMAsIII。不需要样品提取，因此限制了分析前的MAsIII或DMAsIII氧化。检测限低于6 ng As/g组织，使该方法甚至适用于检查低暴露于iAs的研究。
Growing evidence suggest that the methylated trivalent metabolites of inorganic arsenic (iAs), methylarsonite (MAsIII) and dimethylarsinite (DMAsIII), contribute to adverse effects of iAs exposure. However, the lack of suitable methods has hindered the quantitative analysis of MAsIII and DMAsIII in complex biological matrices. Here, we show that hydride generation-cryotraging-atomic absorption spectrometry can quantify both MAsIII and DMAsIII in livers of mice exposed to iAs. No sample extraction is required, thus limiting MAsIII or DMAsIII oxidation prior to analysis. The limits of detection are below 6 ng As/g of tissue, making this method suitable even for studies examining low exposures to iAs.