XOTCH INHIBITS CELL-DIFFERENTIATION IN THE XENOPUS RETINA
XOTCH INHIBITS CELL-DIFFERENTIATION IN THE XENOPUS RETINA
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DOI：
10.1016/0896-6273(95)90305-4
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发表时间：
1995-03-01
期刊：
影响因子：
16.2
通讯作者：
HARRIS, WA
中科院分区：
文献类型：
--
作者：
DORSKY, RI;RAPAPORT, DH;HARRIS, WA
神经基因Xotch在非洲爪蟾胚胎原肠胚形成过程中起着转移细胞决定的作用。我们研究了Xotch在发育中的视网膜中的作用，其中细胞信号传导事件被认为会影响分化。Xotch在睫状边缘区和晚期胚胎中央视网膜的未分化前体细胞中表达。在干细胞或分化的神经元和神经胶质中不表达。即使在没有细胞分裂的情况下，视网膜中的表达也是空间受限的。中央视网膜中的最终Xotch阳性前体细胞大多分化为Muller胶质细胞，这表明这是青蛙视网膜中细胞的最后可用命运。将激活形式的Xotch转染到分离的视网膜细胞中导致它们保留神经上皮形态，表明Xotch的持续激活抑制细胞分化。
The neurogenic gene Xotch acts to divert cellular determination during gastrulation in Xenopus embryos. We examined the role of Xotch in the developing retina, where cell signaling events are thought to affect differentiation. Xotch is expressed in undifferentiated precursor cells of the ciliary marginal zone and late embryonic central retina. It is not expressed in stem cells or in differentiated neurons and glia. Expression in the retina is spatially restricted even in the absence of cell division. The final Xotch-positive precursor cells in the central retina mostly differentiate as Muller glia, suggesting that this is the last available fate of cells in the frog retina. Transfection of an activated form of Xotch into isolated retinal cells causes them to retain a neuroepithelial morphology, indicating that the continued activation of Xotch inhibits cell differentiation.