Serine Resolvases.
Serine Resolvases.
复制标题
DOI：
10.1128/microbiolspec.mdna3-0045-2014
复制
发表时间：
2015-04
影响因子：
3.7
通讯作者：
Rice PA
中科院分区：
文献类型：
--
作者：
Rice PA
丝氨酸分解酶是一组有趣的位点特异性重组酶，在它们的原生环境中，将大的融合复制子分解成较小的分离复制子。一些分解酶由复制转座子编码并分解转座子产物，其中供体和受体分子融合为单独的复制子。其他分解酶由质粒编码，其功能是将质粒二聚体分解成单体。因此，这两种类型都与抗生素抗性基因的传播和维持有关。分解酶和密切相关的转化酶是第一批被详细研究的丝氨酸重组酶，我们对丝氨酸重组酶不寻常的链交换机制的理解很大程度上归功于这些早期的研究。分解酶和转化酶也作为理解DNA拓扑结构如何被利用来调节酶活性的范例。最后，它们相对模块化的结构，结合丰富的结构和生化数据，使它们成为具有设计特异性的工程嵌合重组酶的良好选择。本章重点介绍了目前对丝氨酸分解的理解，重点介绍了结构研究的贡献。
Serine resolvases are an interesting group of site-specific recombinases that, in their native contexts, resolve large fused replicons into smaller separated ones. Some resolvases are encoded by replicative transposons and resolve the transposition product, in which the donor and recipient molecules are fused, into separate replicons. Other resolvases are encoded by plasmids and function to resolve plasmid dimers into monomers. Both types are therefore involved in the spread and maintenance of antibiotic-resistance genes. Resolvases and the closely related invertases were the first serine recombinases to be studied in detail, and much of our understanding of the unusual strand exchange mechanism of serine recombinases is owed to those early studies. Resolvases and invertases have also served as paradigms for understanding how DNA topology can be harnessed to regulate enzyme activity. Finally, their relatively modular structure, combined with a wealth of structural and biochemical data, has made them good choices for engineering chimeric recombinases with designer specificity. This chapter focuses on the current understanding of serine resolvases, with a focus on the contributions of structural studies.