Difference in plasma metabolite concentration after ingestion of lemon flavonoids and their aglycones in humans
Difference in plasma metabolite concentration after ingestion of lemon flavonoids and their aglycones in humans
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DOI：
10.3177/jnsv.52.54
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发表时间：
2006-02-01
影响因子：
1.6
通讯作者：
Kondo, K
中科院分区：
文献类型：
--
作者：
Miyake, Y;Sakurai, C;Kondo, K
研究人员摄入柠檬中的黄烷酮苷（FG）及其苷元（EA）后，检测了人血浆中代谢物的浓度。从柠檬皮中制备出富含圣草次苷的FG，并通过β-葡萄糖苷酶处理从FG中制备出富含圣草酚的FA。受试者服用 FG 和水或 EA 和水后 4 小时内，血浆中的圣草酚、高圣草酚和橙皮素在非酶治疗的血浆中未检测到，但在用 β-葡萄糖醛酸酶和硫酸酯酶治疗后的血浆中检测不到。人类摄入 FG 和 FA 后血浆中的代谢物以圣草酚、高圣草酚和橙皮素的葡萄糖醛和/或磺基结合物的形式存在。摄入FA后，血浆中代谢物的浓度在1小时内出现最高峰值。 FA代谢物的AUC（血药浓度时间曲线下面积）水平高于FG。 FA 被认为比 FG 吸收更快、吸收量更高。与服用水的 FG 相比，服用含类黄酮柠檬汁的 FG 后，受试者血浆中代谢物的 AUC 降低至较低水平。当受试者摄入 FA 和伏特加（40% 乙醇）时，血浆中代谢物的最大浓度峰值在 0.5 小时比 FA 和水更快，但 AUC 水平与 EA 和水相似。 FG 和 EA 的吸收时间受到共存溶液的影响。
The concentrations of metabolites in human plasma after ingestion of flavanone glycosides (FG) and their aglycones (EA) in lemon were examined. FG consisting abundantly of eriocitrin were prepared from lemon peel and FA consisting abundantly of eriodictyol were prepared from FG by treatment with beta-glucosidase. Eriodictyol, homoeriodictyol, and hesperetin in plasma up to 4 h after ingestion of FG with water or EA with water by subjects were not detected in plasma of non-enzyme treatment but in plasma after treatment with beta-glucronidase and sulfatase. Metabolites in plasma after ingestion of FG and FA in humans were shown to exist as the glucuro- and/or sulfo-conjugates of eriodictyol, homoeriodictyol, and hesperetin. After ingestion of FA, the concentration of metabolites in plasma exhibited a high maximum peak at 1 h. The AUC (area under the blood concentration time curve) level of metabolites of FA was higher than that of FG. FA were suggested to be absorbed faster and in higher amounts than FG. The AUC of metabolites in subject plasma after ingestion of FG with flavonoid-depleted lemon juice was shown to change to a low level in comparison with that of FG with water. The maximum concentration peak of metabolites in plasma was faster at 0.5 h than FA with water but the AUC level was similar to EA with water, when subjects ingested FA with vodka (40% ethanol). The absorption hour of FG and EA was shown to be affected by the co-existing solution.