Monoclonal antibodies to murine immunoglobulin isotypes.
Monoclonal antibodies to murine immunoglobulin isotypes.
复制标题
鼠免疫球蛋白同种型的单克隆抗体。
作者：
Weiss,S;Lehmann,K;Cohn,M
我们通过将小鼠骨髓瘤P3X63Ag8.653与免疫了几种含λ1骨髓瘤蛋白的Lewis大鼠脾细胞融合，获得了产生μ、α、λ和λ1单克隆抗体的细胞系。抗λ1抗体特别有用。结果表明，它适用于放射免疫测定，当与碱性磷酸酶偶联时，适用于elisa，适用于膜和细胞质的间接荧光染色。
We have obtained cell lines producing monoclonal antibodies against μ, α, λ, and λ1by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several λ1bearing myeloma proteins. The anti-λ1antibody is particularly useful. It was shown to be suitable for radioimmunoassays, for ELISAs when coupled to alkaline phosphatase, and for indirect fluorescence staining of the membrane as well as the cytoplasm.