Molecular identification of Plasmodium species in symptomatic children of Democratic Republic of Congo.
Molecular identification of Plasmodium species in symptomatic children of Democratic Republic of Congo.
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DOI：
10.1186/s12936-018-2480-5
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发表时间：
2018-09-20
期刊：
影响因子：
3
通讯作者：
Muyembe JJ
中科院分区：
文献类型：
--
作者：
Kavunga-Membo H;Ilombe G;Masumu J;Matangila J;Imponge J;Manzambi E;Wastenga F;Ngoyi DM;Van Geetruyden JP;Muyembe JJ
在世界范围内，疟疾死亡率最高的是恶性疟原虫感染。然而，其他种类的疟原虫（间日疟原虫、卵形疟原虫、三日疟原虫和诺氏疟原虫）也可引起疟疾。因此，准确识别疟疾种类对于患者管理和流行病学监测至关重要。本研究的目的是确定不同的疟原虫物种引起疟疾的5岁以下的儿童在两个省（金沙萨和北基伍省）的刚果（DRC）。2015年10月至12月，在金沙萨和北基伍的综合参考医院进行了一项基于卫生设施的横断面研究。从≤ 5岁的发热儿童中采集了407份血液样本。进行巢式聚合酶链反应测定用于疟原虫种属鉴定。在407名儿童中，142名（34.9%）感染了疟原虫。恶性疟原虫占99.2%。在这些受感染的儿童中，124人感染了恶性疟原虫，1人感染了三日疟原虫。恶性疟原虫/三日疟原虫混合感染6例（1.5%），恶性疟原虫/间日疟原虫混合感染8例（2.0%）。女性感染率（64.8%）高于男性（35.2%），p < 0.001。感染的年龄特异性分布显示，与2岁以上的儿童（81.6%）相比，2岁以下的儿童感染较少（18.4%），p < 0.001。虽然这项研究清楚地表明，最流行的物种是恶性疟原虫，但研究结果表明，在刚果民主共和国金沙萨省和北基伍省5岁以下儿童中存在非恶性疟原虫疟疾，特别是三日疟原虫和间日疟原虫。
Worldwide, the highest malaria mortality is due to Plasmodium falciparum infection. However, other species of Plasmodium (Plasmodium vivax, Plasmodium ovale, Plasmodium malariae, and Plasmodium knowlesi) can also cause malaria. Therefore, accurate identification of malaria species is crucial for patient management and epidemiological surveillance. This study aimed to determine the different Plasmodium species causing malaria in children under 5 years old in two provinces (Kinshasa and North Kivu) of the Democratic Republic of Congo (DRC). From October to December 2015, a health-facility based cross-sectional study was conducted in General Reference Hospitals in Kinshasa and North Kivu. Four hundred and seven blood samples were collected from febrile children aged ≤ 5 years. Nested polymerase chain reaction assays were performed for Plasmodium species identification. Out of 407 children, 142 (34.9%) were infected with Plasmodium spp. and P. falciparum was the most prevalent species (99.2%). Among those infected children, 124 had a mono infection with P. falciparum and one with P. malariae. Mixed infections with P. falciparum/P. malariae and P. falciparum/P. vivax were observed in 6 (1.5%) and 8 (2.0%) children, respectively. The prevalence of infection was higher in females (64.8%) than in males (35.2%), p < 0.001. The age-specific distribution of infection showed that children of less than 2 years old were less infected (18.4%) compared to those aged above 2 years (81.6%), p < 0.001. Although this study showed clearly that the most prevalent species identified was P. falciparum, the findings demonstrate the existence of non-falciparum malaria, especially P. malariae and P. vivax among children aged ≤ 5 years living both Kinshasa and North Kivu Provinces in DRC.
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DOI：
10.3347/kjp.2015.53.5.571
发表时间：
2015-10
期刊：
The Korean journal of parasitology
影响因子：
--
作者：
Kim MJ;Jung BK;Chai JY;Eom KS;Yong TS;Min DY;Siza JE;Kaatano GM;Kuboza J;Mnyeshi P;Changalucha JM;Ko Y;Chang SY;Rim HJ
通讯作者：
Rim HJ
影响因子：
3
作者：
Williams J;Njie F;Cairns M;Bojang K;Coulibaly SO;Kayentao K;Abubakar I;Akor F;Mohammed K;Bationo R;Dabira E;Soulama A;Djimdé M;Guirou E;Awine T;Quaye SL;Ordi J;Doumbo O;Hodgson A;Oduro A;Magnussen P;Ter Kuile FO;Woukeu A;Milligan P;Tagbor H;Greenwood B;Chandramohan D
通讯作者：
Chandramohan D
影响因子：
3
作者：
Ferrari G;Ntuku HM;Schmidlin S;Diboulo E;Tshefu AK;Lengeler C
通讯作者：
Lengeler C
影响因子：
1.2
作者：
Musapa, Mulenga;Kumwenda, Taida;Mharakurwa, Sungano
通讯作者：
Mharakurwa, Sungano
影响因子：
3
作者：
Fru-Cho, Jerome;Bumah, Violet V.;Haldar, Kasturi
通讯作者：
Haldar, Kasturi