Bioassay and molecular detection of insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia
Bioassay and molecular detection of insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia
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印度尼西亚中爪哇省登革热媒介埃及伊蚊对杀虫剂耐药性的生物测定和分子检测
DOI：
10.13057/biodiv/d240136
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
A. Budiharjo
中科院分区：
文献类型：
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作者：
S. Sayono;U. Nurullita;Wahyu Handoyo;Winda Septy Tyasningrum;Irfanul Chakim;A. Budiharjo
抽象的。首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容印度尼西亚中爪哇省登革热媒介埃及伊蚊抗药性的生物测定和分子检测生物多样性24：300-307。埃及伊蚊种群中出现抗药性菌株阻碍了流行地区的登革热控制计划。此外，了解Ae的抗药性现状和分布情况。利用氯氰菊酯、马拉硫磷和敌敌畏等化合物，对印度尼西亚中爪哇省登革热流行区进行了形态和分子检测。从代表不同海拔的5个登革热流行区13个村采集蚊幼虫。幼虫期和成虫期。每个村庄的埃及伊蚊种群都接受了基于世卫组织程序的生物化验。随后，采集样本并进行分子分析，利用等位基因特异性聚合酶链式反应(AS-PCR)鉴定1016G kdr等位基因。Ae.死亡率。接触氯氰菊酯、马拉硫磷和敌敌畏后埃及伊蚊的感染率分别为16%-86%、75%-100%和6%-51%。这些发现表明，Ae.埃及伊蚊种群对氯氰菊酯和敌敌畏具有抗性，但在23.08%的不同海拔地区发现马拉硫磷敏感菌株。AS-PCR结果表明，Aanav基因1016密码子的纯合子(G/G)和杂合子(V/G)等位基因在整个研究地点都存在。Ae中存在多重耐药株。印度尼西亚中爪哇省的埃及伊蚊种群。氯氰菊酯和敌敌畏化合物的使用必须推迟至少五年，而马拉硫磷仍然可以有选择地使用来控制Ae。埃及人在几个地区居住，即Karangjati、Gebugan(Semarang区)和Semarang市的Rowosari。
Abstract. Sayono, Nurullita U, Handoyo W, Tyasningrum WS, Chakim I, Budiharjo A. 2023. Bioassay and molecular detectionsof insecticides resistance of Aedes aegypti, vector of dengue in Central Java Province, Indonesia. Biodiversitas 24: 300-307. The emergence of insecticide-resistant strains among Aedes aegyptipopulations hampered Dengue control programs in the endemic areas. Moreover, to understand the current situation and distribution of insecticide resistance status of Ae. aegypti to cypermethrin, malathion, and temephos compounds, we conducted morphological and molecular detection in the Dengue endemic areas in Central Java Province, Indonesia. Mosquito larvae were obtained from thirteen villages of five Dengue endemic areas representing different altitudes. Larval and adult stage of Ae. aegypti colony from each village was subjected to a bioassay test based on the WHO procedures. Subsequently, they were sampled and subjected to molecular analysis to identify the 1016G kdr allele using the allele-specific polymerase chain reaction (AS-PCR). Mortality of Ae. aegypti after exposure to cypermethrin, malathion, and temephos ranged from 16-86%, 75-100%, and 6-51%, respectively. These findings showed that Ae. aegypti populations were resistant to cypermethrin and temephos, although malathion-susceptible strains were found among 23.08% of the different altitudinal localities. The result of the AS-PCR indicated that the homozygous (G/G) and heterozygous (V/G) alleles of codon 1016 of the AaNav gene were found throughout the study site altitudes. The development of multiple resistance strains was found among Ae. aegypti populations in Central Java Province, Indonesia. The use ofcypermethrin and temephos compounds must be delayed for at least five years, while malathion can still be used selectively to control the Ae. aegypti population in several areas, namely Karangjati, Gebugan (Semarang District), and Rowosari in Semarang City.