ISOLATION AND CHARACTERIZATION OF A CDNA AND GENE CODING FOR A 4TH CD1 MOLECULE
ISOLATION AND CHARACTERIZATION OF A CDNA AND GENE CODING FOR A 4TH CD1 MOLECULE
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DOI：
10.1073/pnas.86.1.252
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发表时间：
1989-01-01
影响因子：
11.1
通讯作者：
TERHORST, C
中科院分区：
文献类型：
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作者：
BALK, SP;BLEICHER, PA;TERHORST, C
CD1基因座编码一个主要组织相容性复合体（MHC）抗原样糖蛋白家族，其与β2 -微球蛋白相关联，并在未成熟的胸腺细胞和朗格汉斯细胞上表达。通过单克隆抗体和分子克隆已鉴定出三种CD1分子：CD1a、CD1b和CD1c。我们从人胸腺细胞文库中分离出了一种编码第四种CD1分子的cDNA，并将该分子命名为CD1d。本文报道了CD1d的完整核苷酸序列和基因组结构。它们表明该分子与先前鉴定的CD1a、CD1b和CD1c分子以及MHC I类分子相关，具有三个胞外结构域、一个跨膜结构域和一个短的胞质尾。CD1d的序列在膜远端的α1和α2结构域以及5′非翻译区在CD1分子中差异最大。相比之下，所有四种CD1分子在膜近端的α3结构域高度同源，该结构域可能参与β2 -微球蛋白的结合。对CD1和MHC I类序列的比较表明，这些分子各自进化以与一组不同的细胞表面蛋白相互作用。
The CD1 locus encodes a family of major histocompatibility complex (MHC) antigen-like glycoproteins which associate with .beta.2-microglobulin and are expressed on immature thymocytes and Langerhans cells. Three CD1 molecules have been identified by monoclonal antibodies and molecular cloning: CD1a, -b, and -c. We have isolated a cDNA coding for a fourth CD1 molecule from a human thymocyte library and termed this molecule CD1d. Reported here are the complete nucleotide sequence and genomic organization of CD1d. They predict that this molecule is related to the previously identified CD1a, -b, and -c molecules and to MHC class I molecules, with three external domains, a transmembrane domain, and a short cytoplasmic tail. The sequence of CD1d is the most divergent among the CD1 molecules in the membrane-distal .alpha.1 and .alpha.2 domains and in the 5'' untranslated region. In contrast, all four CD1 molecules are highly homologous in the membrane-proximal .alpha.3 doamin, which is likely involved in .beta.2-microglobulin binding. A comparison of CD1 and MHC class I sequences suggests that these molecules each evolved to interact with a distinct set of cell surface proteins.