PYRIDINE NUCLEOSIDASES FROM BACILLUS-SUBTILIS AND NEUROSPORA-CRASSA - ISOLATION AND STRUCTURAL-PROPERTIES
PYRIDINE NUCLEOSIDASES FROM BACILLUS-SUBTILIS AND NEUROSPORA-CRASSA - ISOLATION AND STRUCTURAL-PROPERTIES
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DOI：
10.1016/0003-9861(75)90215-5
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发表时间：
1975-01-01
影响因子：
3.9
通讯作者：
KAPLAN, NO
中科院分区：
文献类型：
--
作者：
EVERSE, J;EVERSE, KE;KAPLAN, NO
枯草芽孢杆菌和粗糙脉孢霉的DPNase以及DPNase抑制剂发现了INB。使用常规技术将枯草杆菌纯化至均一。这三种化合物的不同寻常之处在于它们含有大量的碳水化合物，主要由半乳糖、甘露糖和氨基糖组成。B中的碳水化合物部分。枯草杆菌DPNase。抑制物和神经孢子酶分别占分子量的55%、71%和76%。枯草杆菌脱氧核糖核酸酶完全被化学计量比的抑制剂抑制。该酶-抑制物复合体能够结合DPn+，但不能水解二核苷酸。枯草杆菌DPNase。
The DPNases fromBacillus subtilisandNeurospora crassaas well as the DPNase inhibitor found inB. subtiliswere purified to homogeneity using conventional techniques. The three compounds are unusual in that they contain large amounts of carbohydrate, consisting chiefly of galactose, mannose, and aminosugars. The carbohydrate portions in theB. subtilisDPNase. the inhibitor, and theNeurosporaenzyme represent 55, 71, and 76%, respectively, of the molecular weights.TheB. subtilisDPNase is completely inhibited by a stoichiometric amount of the inhibitor. The enzyme-inhibitor complex is capable of binding DPN+, but cannot hydrolyze the dinucleotide.Some preliminary experiments are described using affinity chromatography for the purification of theB. subtilisDPNase.