The commonly used marker ELAV is transiently expressed in neuroblasts and glial cells in the Drosophila embryonic CNS
The commonly used marker ELAV is transiently expressed in neuroblasts and glial cells in the Drosophila embryonic CNS
复制标题
DOI：
10.1002/dvdy.21372
复制
发表时间：
2007-12-01
影响因子：
2.5
通讯作者：
Technau, Gerhard M.
中科院分区：
文献类型：
--
作者：
Berger, Christian;Renner, Simone;Technau, Gerhard M.
果蝇胚胎神经系统中的胶质细胞可以用标记物反向极性 (Repo) 进行监测，而神经元缺乏 Repo 并表达 RNA 结合蛋白 ELAV（胚胎致死，异常视力）。自 1991 年首次描述 ELAV 蛋白分布（Robinow 和 White）以来，人们相信 ELAV 是一种独特的神经元和有丝分裂后标记。通过观察 ELAV 的表达，我们意外地发现，除了神经元之外，ELAV 在胚胎神经胶质细胞中也瞬时表达。此外，它短暂存在于增殖的纵向成胶质细胞中，并在胚胎神经母细胞中转录。同样，通常用作有丝分裂后神经元驱动细胞系的 elav-Gal4 细胞系在神经祖细胞和几乎所有胚胎神经胶质细胞中都有表达。因此，在胚胎中，elav 不再被认为是有丝分裂后神经元的唯一标记或驱动因素。 elav 功能丧失突变体对胚胎神经胶质细胞的数量和模式没有明显影响。
Glial cells in the Drosophila embryonic nervous system can be monitored with the marker Reversed-polarity (Repo), whereas neurons lack Repo and express the RNA-binding protein ELAV (Embryonic Lethal, Abnormal Vision). Since the first description of the ELAV protein distribution in 1991 (Robinow and White), it is believed that ELAV is an exclusive neuronal and postmitotic marker. Looking at ELAV expression, we unexpectedly observed that, in addition to neurons, ELAV is transiently expressed in embryonic glial cells. Furthermore, it is transiently present in the proliferating longitudinal glioblast, and it is transcribed in embryonic neuroblasts. Likewise, elav-Gal4 lines, which are generally used as postmitotic neuronal driver lines, show expression in neural progenitor cells and nearly all embryonic glial cells. Thus, in the embryo, elav can no longer be considered an exclusive marker or driver for postmitotic neurons. elav loss-of-function mutants show no obvious effects on the number and pattern of embryonic glia.