Lunx is a superior molecular marker for detection of non-small lung cell cancer in peripheral blood
Lunx is a superior molecular marker for detection of non-small lung cell cancer in peripheral blood
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DOI：
10.1016/s1525-1578(10)60480-1
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发表时间：
2003-11-01
影响因子：
4.1
通讯作者：
Gillanders, WE
中科院分区：
文献类型：
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作者：
Mitas, M;Hoover, L;Gillanders, WE
对非小细胞肺癌（NSCLC）的临床治疗大有裨益。一种能够检测外周血中少量非小细胞肺癌的检测方法。在本报告中，我们采用了一种新的策略，从24例I至IV期NSCLC患者的外周血中富集肿瘤细胞，并测定了6种癌症相关基因（lunx、muc1、KS1/4、CEA、CK19和PSE）的表达水平。使用高于15例正常对照平均值3个标准差的阈值，我们观察到从NSCLC患者获得的24份外周血样本中有14份（58%）过表达lunx（24份中有10份，42%）、muc1（24份中有5份，21%）和CK19（24份中有5份，21%）。过表达KS1/4 （n = 2）或PSE （n = 1）的患者也过表达lunx或muc1。在假定可治愈和可切除的I至II期疾病患者（n = 7）中，3例（43%）患者中至少有一种标志物过表达。在晚期III至IV期患者（n = 17）中，11例患者（65%）至少有一种标志物过表达。这些结果提供了高通量分子技术可以检测非小细胞肺癌患者循环肿瘤细胞的证据。需要进一步的研究来确定基因过表达的临床相关性。
The clinical management of non-small cell lung cancer (NSCLC) would benefit greatly by. a test that was able to detect small amounts of NSCLC in the peripheral blood. in this report, we used a novel strategy to enrich tumor cells from the peripheral blood of 24 stage I to IV NSCLC patients and determined expression levels for six cancer-associated genes (lunx, muc1, KS1/4, CEA, CK19, and PSE). Using thresholds established at three standard deviations above the mean observed in 15 normal controls, we observed that lunx (10 of 24, 42%), muc1 (5 of 24, 21%), and CK19 (5 of 24, 21%) were overexpressed in 14 of 24 (58%) peripheral blood samples obtained from NSCLC patients. Patients who overexpressed either KS1/4 (n = 2) or PSE (n = 1) also overexpressed either lunx or muc1. Of patients with presumed curable and resectable stage I to II disease (n = 7), at least one marker was overexpressed in three (43%) patients. in advanced stage III to IV patients (n = 17), at least one marker was overexpressed in 11 patients (65%). These results provide evidence that circulating tumor cells can he detected in NSCLC patients by a high throughput molecular technique. Further studies are needed to determine the clinical relevance of gene overexpression.