Interaction of Candida albicans with an Intestinal Pathogen, Salmonella enterica Serovar Typhimurium
Interaction of Candida albicans with an Intestinal Pathogen, Salmonella enterica Serovar Typhimurium
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DOI：
10.1128/ec.00016-09
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发表时间：
2009-05-01
期刊：
影响因子：
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通讯作者：
Mylonakis, Eleftherios
中科院分区：
文献类型：
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作者：
Tampakakis, Emmanouil;Peleg, Anton Y.;Mylonakis, Eleftherios
白色念珠菌是一种机会性的人类真菌病原体，通常存在于胃肠道和皮肤上，但可引起危及生命的侵袭性疾病。鼠伤寒沙门氏菌（Salmonella enterica serovar Typhimurium）是一种革兰氏阴性细菌病原体，可引起人类大量胃肠道感染。这两种微生物对秀丽隐杆线虫也是致病的，引起持续的肠道感染，导致蠕虫死亡。在本研究中，我们使用了以前开发的C。elegans多微生物感染模型，以评估S. Typhimurium和C.白色念珠菌我们观察到当C. elegans感染了C.白念珠菌和鼠伤寒血清型、C.抑制白色念珠菌的增殖。C.白色念珠菌（S. albicans）为优势菌。C. elegans似乎是由一种分泌分子介导的，因为过滤灭菌的细菌上清液能够抑制C.白色念珠菌体外共培养实验表明，S。鼠伤寒沙门氏菌降低了C.白色念珠菌，在37 ℃下观察到的效果比在30 ℃下观察到的效果大。有趣的是，S。鼠伤寒沙门氏菌降低了酵母和丝状形式的C。白色念珠菌，但杀死似乎更迅速的丝状细胞。在C.白色念珠菌生物膜环境。这项研究描述了两种不同的人类病原体之间的相互作用，居住在胃肠道，并表明，原核生物，S。鼠伤寒沙门氏菌降低了真核生物C.白色念珠菌确定这种相互作用的分子机制可能为微生物发病机制提供重要的见解。
Candida albicans is an opportunistic human fungal pathogen that normally resides in the gastrointestinal tract and on the skin as a commensal but can cause life-threatening invasive disease. Salmonella enterica serovar Typhimurium is a gram-negative bacterial pathogen that causes a significant amount of gastrointestinal infection in humans. Both of these organisms are also pathogenic to the nematode Caenorhabditis elegans, causing a persistent gut infection leading to worm death. In the present study, we used a previously developed C. elegans polymicrobial infection model to assess the interactions between S. Typhimurium and C. albicans. We observed that when C. elegans is infected with C. albicans and serovar Typhimurium, C. albicans filamentation is inhibited. The inhibition of C. albicans filamentation by S. Typhimurium in C. elegans appeared to be mediated by a secretary molecule, since filter-sterilized bacterial supernatant was able to inhibit C. albicans filamentation. In vitro coculture assays under planktonic conditions showed that S. Typhimurium reduces the viability of C. albicans, with greater effects seen at 37 C than at 30 C. Interestingly, S. Typhimurium reduces the viability of both yeast and filamentous forms of C. albicans, but the killing appeared more rapid for the filamentous cells. The antagonistic interaction was also observed in a C. albicans biofilm environment. This study describes the interaction between two diverse human pathogens that reside within the gastrointestinal tract and shows that the prokaryote, S. Typhimurium, reduces the viability of the eukaryote, C. albicans. Identifying the molecular mechanisms of this interaction may provide important insights into microbial pathogenesis.