ELECTROCHEMICAL, PHARMACOLOGICAL AND ELECTROPHYSIOLOGICAL EVIDENCE OF RAPID DOPAMINE RELEASE AND REMOVAL IN THE RAT CAUDATE-NUCLEUS FOLLOWING ELECTRICAL-STIMULATION OF THE MEDIAN FOREBRAIN-BUNDLE
ELECTROCHEMICAL, PHARMACOLOGICAL AND ELECTROPHYSIOLOGICAL EVIDENCE OF RAPID DOPAMINE RELEASE AND REMOVAL IN THE RAT CAUDATE-NUCLEUS FOLLOWING ELECTRICAL-STIMULATION OF THE MEDIAN FOREBRAIN-BUNDLE
复制标题
DOI：
10.1016/0014-2999(85)90394-2
复制
发表时间：
1985-01-01
影响因子：
5
通讯作者：
WIGHTMAN, RM
中科院分区：
文献类型：
--
作者：
MILLAR, J;STAMFORD, JA;WIGHTMAN, RM
快速循环伏安法用于监测中前脑束电刺激后纹状体中电活性物质的释放。通过电化学、药理学和神经生理学手段表明释放物质是多巴胺。该材料的伏安图与离子电渗应用的多巴胺相同，但与 DOPAC [二羟基苯乙酸]不同。左旋多巴（多巴胺的代谢前体）可增加释放。 NSD 1015 [3-羟基苯甲基肼]（一种多巴脱羧酶抑制剂）和α-甲基-对酪氨酸（一种酪氨酸羟化酶抑制剂）减少释放。利血平会破坏囊泡多巴胺的储存，从而消除多巴胺的释放。对氯苯丙氨酸是一种色氨酸羟化酶抑制剂，没有效果。方波刺激仅在使用长于 0.5 毫秒的脉冲时才有效。这表明对非常细的无髓鞘纤维的刺激，与黑质纹状体多巴胺纤维的已知形态一致。
Fast cyclic voltammetry was used to monitor the release of electroactive material in the striatum following electrical stimulation of the median forebrain bundle. The release material was shown to be dopamine by electrochemical, pharmacological and neurophysiological means. The material gave a voltammogram identical to that of iontophoretically applied dopamine but not DOPAC [dihydroxyphenylacetic acid]. Release was increased by L-dopa, the metabolic precursor of dopamine. NSD 1015 [3-hydroxybenzylhydrazine] an inhibitor of dopa decarboxylase, and .alpha.-methyl-p-tyrosine, a tyrosine hydroxylase inhibitor, decreased release. Reserpine, which disrupts vesicular dopamine storage, abolished release. Parachlorophenylalanine, a tryptophan hydroxylase inhibitor, had no effect. Square wave stimulation was only effective when pulses longer than 0.5 ms were used. This indicated stimulation of very fine unmyelinated fibers, consistent with the known morphology of nigrostriatal dopamine fibers.