Polyclonal Antibodies to Grain Gliadins and Their Application in Wheat Quality Prediction
Polyclonal Antibodies to Grain Gliadins and Their Application in Wheat Quality Prediction
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DOI：
10.1556/crc.36.2008.1.12
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发表时间：
2008-02
影响因子：
1.6
通讯作者：
Q. Li;K. Ji;X. Song;E. Zhang;Y. Pei;Yueming Yan
中科院分区：
文献类型：
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作者：
Q. Li;K. Ji;X. Song;E. Zhang;Y. Pei;Yueming Yan
从三个小麦品种中提取醇溶蛋白。用制备性聚丙烯酰胺凝胶电泳法从农大99260037(优质)、河南9023(中质)和农大98123(劣质)中分离纯化了α-，β-，γ-，ω-醇溶蛋白。以总醇溶蛋白和α-，β-，γ-，ω-醇溶蛋白为抗原免疫BALB/C小鼠，制备相应的多克隆抗体，分别命名为抗A、抗B、抗C、抗Aα、抗Aβ、抗Aγ和抗Aω。这些多克隆抗体与8个品质性状不同的品种的结合力与小麦的某些品质参数相关。抗农大99260037总醇溶蛋白或γ-、ω-醇溶蛋白抗体与品质指标的相关系数较高。在检测的7个多克隆抗体中，有3个(抗Aγ、抗Aω和抗A)的抗体结合力与面团形成时间、强度、评价值和稳定时间呈显著正或负相关，而与吸水率无显著相关性。这些结果表明，多克隆抗体可用于小麦品质参数的快速预测和筛选。
Gliadins were extracted from three wheat varieties, viz. Nongda 99260037 (good quality), Henan 9023 (media quality) and Nongda 98123 (poor quality), and α-, β-, γ-, ω -gliadins were purified from Nongda 99260037 using preparative SDS-PAGE. The total gliadins and α-, β-, γ-, ω -gliadins were used as antigens to immunise BALB/C mice, and the corresponding polyclonal antibodies were prepared, designated as Anti-A, Anti-B, Anti-C, Anti-A α, Anti-A β, Anti-A γ and Anti-A ω, respectively. Binding of the polyclonal antibodies with 8 varieties, that varied in quality properties, showed correlations with some wheat quality parameters. Correlation coefficients between antibodies against total gliadins or γ -, and ω -gliadin of Nongda 99260037 and quality parameters were higher than other antibodies. Of seven polyclonal antibodies tested, three (Anti-A γ, Anti-A ω and Anti-A) displayed significant positive or negative associations between antibody binding and dough development time, strength, valorimenter value and stability time, but no significant correlations were observed with water absorption. These results suggest that polyclonal antibodies could be used for rapid prediction and screening of wheat quality parameters.