Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada)
Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada)
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DOI：
10.14411/eje.2020.050
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发表时间：
2020-01-01
影响因子：
1.3
通讯作者：
Sota, Teiji
中科院分区：
文献类型：
--
作者：
Karagyan, Gayane;Golub, Natalia;Sota, Teiji
生活在美国东部的周期性蝉属MagicicadaDavis，1925的特征是幼年期长达17或13年，成虫周期性地大量出现。在这一属中，只有7个物种被区分出来，并分为三个种组，Decim，Cassini和Decula。我们对七个种进行了比较细胞遗传学研究，其中6个种为首次研究。所用技术包括标准染色体染色、染色体差异染色技术（C-显带、AgNOR-显带和荧光染料DAPI-染色）和荧光原位杂交（FISH）。所有这些物种都有全动染色体，像其他半翅目。所有物种的核型似乎都非常保守，2n = 19/20（18 + X 0/XX）（雄性/雌性）;一对非常大的常染色体和所有其他染色体构成一个递减的大小系列; X染色体是不可区分的，至少在有丝分裂中期。核仁组织者区（NORs）显示在所有物种的一个中等大小的染色体对，并显示与rDNA簇荧光原位杂交显示。在所有物种中，非常小的C-异染色质块位于不同的染色体上，清楚地使用DAPI，但不太清楚的C-显带。所有物种的染色体都含有端粒的“昆虫型”基序（TTAGG）（n）。
The periodical cicadas of the genus Magicicada Davis, 1925 inhabiting eastern United States are characterized by a long juvenile period of 17 or 13 years and periodical mass emergence of adults. In this genus, only seven species are distinguished and are distributed into three species groups, Decim, Cassini and Decula. We performed comparative cytogenetic study of all seven Magicicada species, and 6 of them were explored for the first time. The techniques used included standard chromosome staining, differential chromosome staining techniques (C-banding, AgNOR-banding and fluorochrome DAPI-staining) and FISH (fluorescence in situ hybridization) with 18S rDNA and (TTAGG)(n)-telomeric probes. All these species have holokinetic chromosomes, like other Hemiptera. Karyotypes of all species appeared to be remarkably conserved with 2n = 19/20 (18 + X0/XX) (male/female); one very large pair of autosomes and all other chromosomes constituting a decreasing size series; X chromosomes were indistinguishable, at least, at mitotic metaphases. The nucleolus organizer regions (NORs) revealed on one of the medium-sized chromosome pairs in all species and were shown to coincide with the rDNA clusters revealed by FISH. In all species, very small blocks of C-heterochromatin were variously located on chromosomes clearly using DAPI but less distinctly by C-banding. The chromosomes of all the species studied were confirmed to contain the "insect type" motif of telomeres (TTAGG)(n).