The Liver MicroRNA Expression Profiles Associated With Chronic Hepatitis C Virus (HCV) Genotype-4 Infection: A Preliminary Study.
The Liver MicroRNA Expression Profiles Associated With Chronic Hepatitis C Virus (HCV) Genotype-4 Infection: A Preliminary Study.
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DOI：
10.5812/hepatmon.33881
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发表时间：
2016-04
影响因子：
0.6
通讯作者：
Abdel-Wahab AH
中科院分区：
文献类型：
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作者：
El-Guendy NM;Helwa R;El-Halawany MS;Abdel Rahman Ali S;Tantawy Aly M;Hasan Alieldin N;Fouad SA;Saeid H;Abdel-Wahab AH
MicroRNA（miRNAs）已被反复证明在包括肝炎、肝硬化和肝癌在内的肝脏病理学中发挥重要作用。埃及是世界上丙型肝炎病毒（HCV）感染率最高的国家，主要涉及基因型-4。在这项研究中，我们试图描述慢性感染的埃及患者中研究不足的HCV基因型4的miRNA谱，以更好地了解该疾病及其并发症，并帮助设计更好的管理方案。我们使用实时定量聚合酶链反应（PCR）分析了从50例诊断为慢性HCV感染的埃及患者收集的新鲜肝活检组织中选定的94种miRNA的表达水平。采用非参数检验分析各miRNA的表达水平及其与入组患者临床病理特征的相关性。我们的研究结果揭示了与正常对照相比，所分析的miRNA的差异表达水平。其中27个miRNAs（包括miR-105、miR-147、miR-149- 3 p和miR-196 b）表达上调，17个miRNAs（包括miR-21、miR-122、miR-199 a-3 p和miR-223）表达下调。miR-95、miR-130 a和miR-142- 5 p水平与血白蛋白水平呈负相关。在重度慢性肝脏炎症中观察到7种miRNA（miR-29 c、miR-30 c、miR-126、miR-145、miR-199 a、miR-199 a-3 p和miR-222）的表达水平升高。在这项研究中发现的几种失调的miRNAs以前与慢性肝脏炎症和肝细胞癌（HCC）发展的风险有关。鉴定的一些检测的miRNAs的表达谱可能为埃及和世界各地的初始患者的治疗和慢性感染HCV患者的管理提供重要的考虑点。
MicroRNAs (miRNAs) have been repeatedly shown to play important roles in liver pathologies, including hepatitis, liver cirrhosis, and liver cancer. Egypt has the highest hepatitis C virus (HCV) infection rate worldwide, predominantly involving genotype-4. In this study, we attempted to characterize the miRNA profile of the poorly studied genotype 4 of HCV in chronically infected Egyptian patients to obtain a better understanding of the disease and its complications and help in the design of better management protocols. We analyzed the expression levels of a selected panel of 94 miRNAs in fresh liver biopsies collected from 50 Egyptian patients diagnosed with chronic HCV infection using quantitative real-time polymerase chain reaction (PCR) assay. Non-parametric tests were used to analyze the expression level of each miRNA and association with the clinicopathological features of enrolled patients in this study. Our results revealed differential expression levels of the analyzed miRNAs compared to the normal controls. Twenty-seven miRNAs (including miR-105, miR-147, miR-149-3p, and miR-196b) showed up-regulation, while 17 miRNAs (including miR-21, miR-122, miR-199a-3p, and miR-223) showed down-regulation. An inverse correlation was observed between levels of miR-95, miR-130a, and miR-142-5p with the blood albumin level. Increased expression levels of seven miRNAs (miR-29c, miR-30c, miR-126, miR-145, miR-199a, miR-199a-3p, and miR-222) were observed with severe chronic hepatic inflammation. Several deregulated miRNAs found in this study have been previously linked to chronic liver inflammation and the risk of hepatocellular carcinoma (HCC) development. The identified expression profiles of some examined miRNAs might offer important points to consider for the treatment of naive patients and the management of chronically infected HCV patients in Egypt and around the world.