ASCORBATE-MEDIATED STIMULATION OF NEUTROPHIL MOTILITY AND LYMPHOCYTE-TRANSFORMATION BY INHIBITION OF THE PEROXIDASE-H2O2-HALIDE SYSTEM INVITRO AND INVIVO
ASCORBATE-MEDIATED STIMULATION OF NEUTROPHIL MOTILITY AND LYMPHOCYTE-TRANSFORMATION BY INHIBITION OF THE PEROXIDASE-H2O2-HALIDE SYSTEM INVITRO AND INVIVO
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DOI：
10.1093/ajcn/34.9.1906
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发表时间：
1981-01-01
影响因子：
7.1
通讯作者：
ANDERSON, R
中科院分区：
文献类型：
--
作者：
ANDERSON, R
在6名正常志愿者单次静脉注射1 g抗坏血酸之前和之后1 h，评估了神经元迁移、吞噬后单磷酸己糖分流活性和髓过氧化物酶介导的摄入白色念珠菌碘化和淋巴细胞有丝分裂原诱导转化。观察到中性粒细胞运动性增加，这与髓过氧化物酶介导的C.白色念珠菌和磷酸己糖分流活性略有增加。淋巴细胞转化也增加。这些活动的变化与血清抗坏血酸水平有关。为了研究抗坏血酸介导的中性粒细胞运动性增加和淋巴细胞转化与过氧化物酶活性降低的关系，将正常人的中性粒细胞和淋巴细胞暴露于辣根过氧化物酶/H2 O2/碘化钠系统中存在和不存在抗坏血酸，并分别检测迁移和增殖反应。暴露于辣根过氧化物酶/H2 O2/卤化物系统导致抑制中性粒细胞运动和淋巴细胞对有丝分裂原的反应。然而，列入抗坏血酸保护中性粒细胞和淋巴细胞的辣根过氧化物酶/H2 O2/卤化物系统的抑制作用。
Neutrophil migration, postphagocytic hexose-monophosphate shunt activity and myeloperoxidase-mediated iodination of ingested Candida albicans and lymphocyte mitogen-induced transformation were assessed in 6 normal volunteers before and 1 h after a single i.v. injection of 1 g ascorbate. Increased neutrophil motility was observed which was associated with decreased myeloperoxidase-mediated iodination of C. albicans and a slight increase in hexose-monophosphate shunt activity. Lymphocyte transformation was also increased. Alterations in these activities were related to serum ascorbate levels. To investigate the relationship of ascorbate-mediated increased neutrophil motility and lymphocyte transformation to decreased peroxidase activity, neutrophils and lymphocytes from normal individuals were exposed to the horseradish peroxidase/H2O2/sodium iodide system in the presence and absence of ascorbate and tested for migratory and proliferative responses, respectively. Exposure to the horseradish peroxidase/H2O2/halide system caused inhibition of neutrophil motility and lymphocyte responsiveness to mitogens. However, inclusion of ascorbate protected both the neutrophils and lymphocytes from the inhibitory effects of the horseradish peroxidase/H2O2/halide system.