Distribution and characterization of pedal peptide immunoreactivity in Aplysia.
Distribution and characterization of pedal peptide immunoreactivity in Aplysia.
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DOI：
10.1002/neu.480210606
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发表时间：
1990-09
期刊：
影响因子：
--
通讯作者：
Wade L. Pearson;P. E. Lloyd
中科院分区：
文献类型：
--
作者：
Wade L. Pearson;P. E. Lloyd
足肽（Pedal peptide，Pep）是一种含量丰富的神经肽。放射免疫测定法（RIA）被开发用于定量组织提取物中的Pep-like免疫反应性（IR-Pep）。IR-Pep在中枢神经系统（CNS）和两种外周组织（大两性管（LHD）和足部）中的浓度非常高。从高压液相色谱（HPLC）的馏分的RIA表明，PEP本身是在这些组织中的每一个主要的免疫反应性物种。在许多其他外周组织中发现较低浓度的Pep。掺入标记的氨基酸表明，PEP合成的LHD，而PEP在脚主要是在中枢神经元合成和运输到脚。IR-Pep通过免疫细胞学进一步定位。所有外周IR-Pep似乎与神经元纤维，最常见的是静脉曲张的轴突。LHD和足的免疫反应性神经支配特别密集，但在其他组织中也观察到阳性染色，包括皮层、鳃、肠和心脏，包括LHD在内的所有组织中的IR-Pep神经支配似乎主要局限于组织的肌肉部分。Pep的应用加速了离体LHD的自发收缩。Pep似乎作为一种递质或神经调质，在许多不同的网站在失智症。
Pedal peptide (Pep) is a very abundant neuropeptide in Aplysia. A radioimmunoassay (RIA) was developed to quantify Pep-like immunoreactivity (IR-Pep) in tissue extracts. IR-Pep was present in very high concentrations in the central nervous system (CNS) and two peripheral tissues: the large hermaphroditic duct (LHD) and the foot. RIA of fractions from high-pressure liquid chromatography (HPLC) indicated that Pep itself was the predominant immunoreactive species in each of these tissues. Lower concentrations of Pep were found in a number of other peripheral tissues. Incorporation of labelled amino acid indicated that Pep was synthesized in the LHD, whereas Pep in the foot was synthesized primarily in central neurons and transported to the foot. IR-Pep was further localized by immunocytology. All peripheral IR-Pep appeared to be associated with neuronal fibers, most commonly varicose axons. Immunoreactive innervation of the LHD and foot was particularly dense but positive staining was also observed in other tissues including tegument, gill, gut, and heart, IR-Pep innervation in all tissues including the LHD appeared to be localized predominantly in muscular portions of the tissue. Spontaneous contractions of isolated LHD were accelerated by the application of Pep. Pep appears to act as a transmitter or neuromodulator at a number of different sites in Aplysia.